Key Experiments in Membrane Trafficking

Question 1

who was George Emil Palade?

Answer 1

Romanian nobel prize winner for discoveries of cell structure and functional organisation

Question 2

who was Marilyn Farquhar?

Answer 2

George Palades wife

gorundbreaking discoveries and established cell biology department at Yale

Question 3

what was the Pulse Chase Experiment?

Answer 3

George Palades nobel prize winning experiment
used pancreatic cells exposed to radioctive lucein residues
tracking protein movement through cells
lead to disocvery of golgi body, trans golgi network and ER

Question 4

who was Randy Schekman?

Answer 4

used yeast to identify cellular tracking components
won nobel prize
used saccharomyces cerevisiae yeast

Question 5

who was James Rothman?

Answer 5

'don't be afraid, be stupid'
discovered NEM (n-ethylmaleimide)

Question 6

what is NEM?

Answer 6

NSF is a NEM sensitive factor

works alongside SNAP and NSF and 20S particle

Question 7

who were Giampietro Schiavo and Cesare Montecucco?

Answer 7

italians
show machinery essential for neurotransmission
groundbreaking research for tetanus and botulism