Endocytosis Flashcards
what are three reasons membrane trafficking is important?
- fundamental to all cell types
- implicated in many diseases
- highly conserved process
what is basic process of the secretory pathway?
DNA->mRNA
made into proteins at ribosome then attached to ER
folding at lumen of ER
golgi complex adds PTMs (cis to trans movement)
trans golgi network packs proteins into vesicles
what are the three core processes of membrane trafficking?
transport vesicles bud from donor compartment
vesicles fuse with target compartment
soluble proteins from donor are transferred into new compartment
how do things move inside the cells?
variety of filaments including microtubules, intermediate filaments, and microfilaments
what are the two types of microtubules?
kinesin and dynein
what is the aim of endocytosis?
move molecules into cell
what are three core components of endocytosis processs?
cargo receptors
cargo molecules
vesicles with adaptin and clathrin
what is adaptin?
links cargo receptors to the coat
describe endocytosis between golgi network and ER
involves coat protein (COP)
ER-> Golgi = COP2
Golgi -> ER = COP1
what are the five stages of claterin mediated endocytosis?
nucleation cargo selection coat assembly scission uncoating
what is adaptor protein 2 (AP2)?
links cargo to coat
collect at PIP2 location and bind PIP2 to cargo
what is clathrin?
the vesicular coat produced by clathrin
what is clathrin structure?
triskeleton of polyhedral cages (heavy and light chains)
what are the two key proteins involved in membrane curvature?
epsin and AP180
and these bind to PIP2
what are the two BAR proteins that sense membrane curvature?
amphiphysin and syndapin
these are homodimers
