Isolasi Plasmid Flashcards

1
Q

Analisis DNA plasmid dapat dilakukan dengan cara

A

Analisis kualitatif dengan elektroforesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Berapa tegangan dan waktu yang digunakan untuk running elektroforesis

A

100 volt dan 28 menit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Berapa jumlah volume RNAse yang digunakan

A

0,2x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Tahapan pencucian plasmid dilakukan dengan menambahkan

A

EtOH 70% 500 mikroL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Tahapan isolasi plasmid secara berurutan

A

resuspensi, lisis, netralisasi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Larutan yang ditambahkan setelah DNA dikeringkan

A

ddH2O

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Jika volume supernatan yang diambil adalah 350 mikroL maka berapa volume penambahan larutan P:C:I

A

350 mikroL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Isolasi plasmid dilakukan dengan menambahkan resuspensi solution yang terdiri dari

A

Tris dan EDTA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Jenis bakteri yang digunakan dan jenis plasmid dan jenis gen

A

E. coli dan pGWB5 dan SOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Jenis larutan yang digunakan dalam isolasi plasmid

A

resuspensi solution, neutralization solution, lysis solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Analisis kuantitatif dilakukan dengan

A

Spektofotometri dengan panjang gelombang 260 nm (DNA), 280 nm (Protein)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Larutan lisis yang digunakan

A

NaOH dan SDS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Larutan netralisasi

A

Potassium Acetate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Prinsip lisis

A

Menghancurkan dinding sel dengan merusak protein penyusunnya

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Prinsip presipitasi

A

Mengendapkan suatu senyawa dengan menambahkan senyawa lain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q
A