DNA Barcoding

Question 1

Mix PCR DNA cendawan

Answer 1

Sampel DNA (100ng/mL) 1mikroL
Master mix PCR 5mikroL
Primer ITS-1 F 0,25mikroL
Primer ITS-4 R 0,25mikroL
ddH2O 3,5mikroL

Question 2

Mix PCR DNA bakteri

Answer 2

Sampel DNA (100ng/mL) 1mikroL
Master mix PCR 5mikroL
Primer 16S F 0,25mikroL
Primer 16s R 0,25mikroL
ddH2O 3,5mikroL

Question 3

Profil PCR untuk bakteri dan cendawan

Answer 3

Pre Dena 95C 5’
Dena 95C 50’’
Ann 51C 2’
Ex 72C 2’
Post ex 72C 5’
Cooling down 4C 10’

Question 4

Mix PCR tembakau transgenik

Answer 4

Sampel DNA (100ng/mL) 1mikroL
Master mix PCR 5mikroL
Primer 35S F 0,25mikroL
Primer B11 R 0,25mikroL
ddH2O 3,5mikroL

Question 5

Master mix nanas

Answer 5

Sampel DNA (100ng/mL) 1mikroL
Master mix PCR 5mikroL
Primer OPA 0,5mikroL
ddH2O 3,5mikroL

Question 6

Profil PCR tembakau

Answer 6

Pre Dena 95C 5’
Dena 95C 30’’
Ann 65C 30’’
Ex 72C 1’
Post ex 72C 10’
Cooling down 12C 10’

Question 7

Profil PCR nanas

Answer 7

Pre Dena 94C 2’
Dena 94C 1’
Ann 37C 1’
Ex 72C 2,5’
Post ex 72C 7’
Cooling down 25C 10’