Introduction to zebrafish Flashcards
When were zebra fish first described?
Dr Francis Buchanan-Hamilton
British East India Trading Company records, 1822
Why are zebrafish used as a model?
70 % of related human protein-coding genes
84 % of human disease counterpart genes
Fully sequenced genome + variety of genetic manipulation tools = Enviable toolbox and endless possibilities
Because of its fully sequenced genome, easy genetic manipulation, high fecundity, external fertilization and rapid development, and nearly transparent embryo, zebrafish are a unique model animal for biomedical research, including studies of biological processes and human diseases
Why are zebrafish more environmental better?
Few Standards in husbandry or aquarium design
Common:
High density, small footprint intensive aquaculture
Biosecurity control
What are advantages and disadvantages to facility design for zebra fish?
Advantage
Recirculating Aquaculture Systems (RAS)
Benefits
Self-contained production systems
Greater control over the culture environment
Increased bio-security
Minimal water exchange ~10% system volume / 24hrs
disadvantage
water quality - biological, chemical and mechanical filtration
What are the pros and cons of enrichment in the facility design of zebrafish
PROS
Enhanced learning + slower growth rate
Reduced anxiety-like behaviour
Mitigate aggressive behaviour in males
Reduced avoidance behaviour + enhanced curiosity
Cons:
Not all enrichment is beneficial
Behavioural benefits vs. life support challenges
Impact on water quality
Cleaning Issues
Potenial health issues
Chemicals leaching from plastic
Potential breeding ground for pathogens and pests
Increased aggression (resource hoarding)
What roles do animal technicians have in looking after zebra fish?
Husbandry
Colony Management
Facility Maintenance
Technical procedures
Provision of the 5 freedoms
Maintenance of regulatory standards and ethical compliance
What specific roles does husbandry require?
Daily mortality checks
Weekly health inspections
Real time essential water quality monitoring
Weekly water quality monitoring
Twice Daily Central Life Support equipment checks
What specific roles does technical procedures require?
IVF
Tagging
Tissue collection for pathology testing or genotyping
What are two methods of aneasthesia and analgesia?
Clove-oil derivative
Immersion aneasthetic
Two most commonly utilised:
Tricaine (ms222)
Aqui-S (Isoeugenol)
What are approval does euthanasia of zebra fish require?
Techniques require local AEC approval
What are common methods of euthanasia in zebra fish?
Aneasthesia overdose
Decapitation
Ice-slurry
