Animal in biomedical research Flashcards
What are the characteristics needed in animals for biomedical research?
Small and easy to handle
Short life span
Short gestation period
Large litter sizes
What are examples of these animals?
- rats
- mice
- zebrafish
- nermatodes
How long does an inbred strain of mice take?
+5 years
When was the first transgenic mouse?
1982
When was the first KO mouse
1989
What do practices want from rodent anaesthesia?
immobilisation
unconsciousness
muscle relaxation
why are rodents routinely anaesthetised?
husbandry and experimental purposes
What causes variability in anaesthetisa
species, strain and individual
What are considerations to keep in mind when anaesthetising rodents
Animal Welfare considerations
Research outcomes and requirements
Animal Ethics Committee approval
State and National regulations (GM?)
Length of anaesthesia required
Depth of Anaesthesia required
What types of anaesthesia are there?
- General Anaesthesia
Gaseous/inhalational
Injectable - Local Anaesthesia
Topical
Injectable
What are the benefits and disadvantages for gaseous anaesthesia ?
Advantages:
Accurate control over depth of anaesthesia
Rapid induction and recovery
Oxygen administration during anaesthesia
Disadvantages:
Specialised equipment necessary
Good ventilation and scavenging equipment necessary
Requires training to use equipment
What are the benefits and disadvantages for general anaesthesia such as injectables?
Advantages:
Minimal equipment
Rapid induction for an intravenous injection
Titratable dosing
Some agents are reversible
Disadvantages:
Predetermined dose
Good restraint necessary
Individual variation
Slow absorption in non-IV routes
what are two main rodents analgesias used?
NSAIDS
opioids
