INTRODUCTION TO BACTERIOLOGY Flashcards
Father of protozoology and bacteriology
Anton van Leeuwenhoek
they lack nuclear membrane and a true nucleus, mitochondria an endoplasmic reticulum, and Golgi bodies.
Prokaryotes
membrane-bound nucleus
Eukaryotes
exist as unicellular organisms of microscopic size, whereas others are multicellular organisms
Eukaryotic parasites
unicellular organisms within the kingdom Protista that obtain their nutrition through ingestion.
Protozoa
cannot produce all of its nutrients
Heterotrophic
hyphae that interweave to form mats
Mycelia
obtain nutrients through absorption
Eukaryotes
fungi that have yeast and mold phase
Dimorphic
multicellular fungi that reproduce asexually
Yeasts
filaments of fungi
Hyphae
filamentous forms that can reproduce asexually and sexually.
Molds
virus that infects and possibly destroys bacterial cells.
Bacteriophage
fusion of host cells into multinucleated infected forms.
Syncytium
genetic makeup of an organism
Genotype
visible changes
Cytopathic cells
complete virus outside a cell
Varion
Orderly classification and grouping of organisms
into categories.
Classification/taxonomy
Nomenclature
Identification
domains of plants and protists
Eukarya
observable traits and functional features of an organism.
Phenotype
named similarly to animals; instead divisions phyla is used
Protists
are placed in the domains Bacteria and Archaea
Prokaryotes
last name
Genus
human clan
Family
human first name
Species
smaller than a typical rbc but larger than a virus
bacteria
based on susceptibility to specific bacterial phages
Phage typing
basis of serologic differences
Serovarieties
basis of phenotypic differences
Subspecies
provides naming assignments for each organisms
Nomenclature
basis of biochemical test result differences
Biovarieties
spores are located at the end
terminal spores
spores are located at the middle
central spores
spores are located near at the end
subterminal spores
largest and most inclusive taxa
Bacteria
Archaea
Eukarya
found in microorganisms that grow under extreme environmental conditions
Archaea
structure of the cell envelope and enzymes of archaea allows them to survive under stressful or extreme conditions
Extremophiles
salt-loving cells
Halophiles
heat – loving cells
Thermophiles
prokaryotic cells that infect eukaryotic hosts
Pathogenic bacteria
inhibit bacterial growth without harming eukaryotic host cells
Antimicrobial agents
inventor of the ultracentrifuge
Theodor Svedberg
bacterias that produce endosphores in response to harsh environmental conditions.
Bacillus and Clostridium
small, dormant, asexual spores that develop inside the bacterial cell as a means of survival, not reproduction.
Endosphores
highly refractile bodies in the cell and are visualized with Gram stain as unstained areas in a cell because of their thick protein coat.
Spores
most commonly used endospore stain
Schaeffer-Fulton stain
causative agent of tetanus
Clostridium tetani
made of phospholipids and proteins and does not contain
sterols.
Prokaryotic plasma membrane
contain sterols, it acts as an osmotic barrier and the location of the electron transport chain, where energy is generated.
Eukaryotic plasma membrane
maintains the shape of the cell and prevents bursting of the cell from high osmotic pressure inside it
Cell wall
principle component of the gram-positive cell wall
Thick peptidoglycan wall
unique to the gram-positive cell wall.
Teichoic acid
Lipoteichoic acid
responsible for producing fever and shock in animals infected with gramnegative bacteria; acts as a barrier to hydrophobic compounds and harmful substances
Lipid A moiety
lack the rigidity of the cell wall, they are seen in various shapes microscopically.
Pleomorphic
waxy layer of glycolipids and fatty acids in the acid-fast cell wall
Mycolic acid
distinct component of fungal cell walls
Chitin
largest bacterium known found in ocean sediment
Thiomargarita namibiensis
bacilli with tapered, pointed ends
Fusiform
species differ in size and shape within a pure culture
Pleomorphic
used in the classification and identification of microorganisms
Stains
most commonly used stain in the clinical microbiology laboratory.
Gram stain
used to stain bacteria that have a high mycolic acid and wax content in their cell walls and do not stain well with traditional bacterial stains
Acid fast stain
fluorochrome dye that stains gram-positive and gram-negative bacteria, living or dead. It binds to the nucleic acid of the cell and is used to locate bacteria in blood cultures and other specimens.
Acridine orange
binds to chitin in fungal cell walls. Used in high-volume laundries to whiten yellow-appearing white cotton and other fabrics.
Calcofluor white
used to screen specimens for acid-fast bacteria
Fluorochrome stain
used as a counterstain in acid-fast staining procedures, used as a simple stain to detect white blood cells in stool samples
Methylene blue
used to stain the cell walls of medically important fungi grown in slide cultures
Lactophenol cotton blue
negative stains used to visualize capsules surrounding certain yeasts
India ink
used to stain bacterial spores.
Endospore stain
primary stain of endospore stain
malachite green
able to grow simply, using carbon dioxide
Autotrophs
require more complex substances for growth; used as both the carbon source and energy source
Heterotrophs
contain nutrients that support the growth of most non-fastidious organisms
Nonselective or nutritive type
contains added growth factors, such as blood, vitamins, and yeast extract.
Enriched mediums
contains additives such as dyes, bile salts, alcohols, acids, and microbial agents that inhibit the growth of some bacteria.
Selective media
allow visualization of metabolic differences between groups or species of bacteria
Differential media
distinguishes between lactose fermenters and nonlactose fermenters
MAC agar
Most pathogenic bacteria grow best at a neutral pH
7.0-7.5
distinguishes between hemolytic and non hemolytic organisms
Sheep blood agar
to recover small numbers of organisms that may be present in a clinical sample
Broth media
used to enhance the growth of group B streptococci
Lim broth
holding medium designed to preserve the viability of microorganisms in the specimen but not allow multiplication
Transport medium
encourage the growth of small numbers of a particular organism while suppressing other bacteria
Enrichment broth
cold temperature (10-20 degrees C)
Psychrophiles
moderate temperatures (20-40 degrees C), grow near the human core body temperature
Mesophiles
high temperatures (50-60 degrees C)
Thermophiles
require oxygen for growth
Obligate aerobes
cannot grow in the presence of oxygen
Obligate anaerobes
can survive in the presence of oxygen but grow poorly and do not use oxygen in metabolism
Aerotolerant anaerobes
less energy yielding than aerobic respiration
Anaerobic respiration
consists of the biochemical reactions bacteria used to break down organic compounds and the reactions they use to synthesize new molecules from smaller subunits.
Microbial metabolism
can grow either with or without oxygen
Facultative anaerobes
require a reduced level of oxygen to grow
Microaerophilic bacteria
efficient energy-generating process in which molecular oxygen is the final electron acceptor.
Aerobic respiration
require an atmosphere enriched with carbon dioxide
Capnophilic bacteria
bacterial growth is replicated by
binary fusion
Alternative to EMP pathway for carbohydrate metabolism
PENTOSE PHOSPHATE PATHWAY
anaerobic process carried out by obligate, facultative, and aerotolerant anaerobes.
Fermentation
most important pathway for the complete oxidation of a substrate
Krebs cycle
Converts glucose-6-phosphate to pyruvate and glyceraldehyde phosphate, which can be funneled into other pathways
ENTNER-DOUDOROFF PATHWAY
Major pathway in conversion of glucose to pyruvate
EMBDEN MEYERHOF PARVAS GLYCOLYTIC PATHWAY