Introduction

Question 1

Histochemistry

Answer 1

Tissue staining

Question 2

A tissue/structure that has affinity to a basic

dye

Answer 2

basophilic

Question 3

Basophilic stain

Answer 3

Hematoxilin

Question 4

A tissue/structure that has affinity to an

acidic dye

Answer 4

acidophilic

Question 5

Acidophilic stain

Answer 5

eosin

Question 6

Often cannot be distinguished in typical H&E staining

Answer 6

Cytoplasmic proteins and extracellular matrix proteins (fibroblast cytoplasm blends with collagen in H&E)

Question 7

Periodic-acid Schiff (PAS)

Answer 7

stains molecules

rich in carbohydrates (glycoproteins, proteoglycans)

Question 8

Silver stains

Answer 8

for reticular fibers (Type 3 Collagen), nerve cell

processes

Question 9

Masson’s trichrome

Answer 9

three-colored stain that

distinguishes cells from their surrounding extracellular matrix

Question 10

Colors in masons trichrome

Answer 10

cytoplasm/keratin - red
nuclei - dark/purple
connective tissue (collagen) - blue

Question 11

metachromasia

Answer 11

change
in color within a cell/tissue due to interaction
between a basic dye (e.g. toluidine blue) and
polyanions

Question 12

What do Mineralized tissues require?

Answer 12

specialized

techniques for microscopic viewing

Question 13

• cracks/tears
• folds
• poor fixation
• sectioning defects

Answer 13

Artifacts