Intro

Question 1

study of tissues of the body and how these tissues arrange to constitute organs

Answer 1

Histology

Question 2

this is also termed as microscopic anatomy

Answer 2

histology

Question 3

this is also termed as microscopic anatomy

Answer 3

histology

Question 4

What do you call a group of cells with interrelated function?

Answer 4

Tissue

Question 5

What are the two components of a tissue?

Answer 5

Cells and ECM (Extracellular Matrix)

Question 6

What are the two components of a tissue?

Answer 6

Cells and ECM (Extracellular Matrix)

Question 7

a process wherein tissue structure is preserved, autolysis is prevented, and pathogenic microorganisms are killed

autolysis = cell death caused by enzymes

Answer 7

fixation

Question 8

the most common fixative is?

Answer 8

formalin

Question 9

If the tissue is calcified, this is the step before dehydration

“unhardening”

Answer 9

Decalcification

Question 10

this is the step wherein tissue is bathed successively through a series of increased concentrations of alcohol sol’ns, ending in 100%, which **removes all water **

Answer 10

dehydration

Question 11

the step wherein alcohol from tissue is removed in organic solvents in which alcohol and paraffin are miscible

Answer 11

clearing

Question 12

process wherein the paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden

Answer 12

embedding

Question 13

this is where the paraffin block is trimmed to expose the tissue for thin slicing

Answer 13

trimming

Question 14

the chemicals used for clearing

Answer 14

xylene and toluene

Question 15

the most commonly used stain

Answer 15

H&E - Hematoxylin and Eosin

Question 16

this stains the DNA in the nucleus, RNA-rich portions of the cytoplasm, and produces a dark blue or purple color

Answer 16

Hematoxylin

Question 17

stains the other cytoplasmic structures pink

Answer 17

Eosin

Question 18

what do you call the negative parts in a tissue that adheres to a basic dye

Answer 18

Basophilic

Adheres to a basic dye = basic lover

Question 19

what do you call the positive parts in a tissue that adheres to an acidic dye

Answer 19

Acidophilic

Acid lovers

Question 20

which among the H&E is acidic? is basic?

Answer 20

Hematoxylin - basic
Eosin - acidic

Thus:
Hematoxylin = for negative parts
Eosin = for positive parts

negative parts = basophilic
positive parts = acidophilic

Question 21

Feulgen test

Answer 21

DNA

Question 22

Periodic Acid Schiff (PAS)

Answer 22

Carbohydrates

Question 23

Sudan Black

Answer 23

Lipids

Question 24

Silver Stain

Answer 24

Reticular Fibers

Question 25

Glycolysis without oxygen results to the production of?

Answer 25

Lactic Acid

Question 26

the method most commonly used in microscopy; uses ordinary light and the colors are imparted by tissue staining

Answer 26

Bright-Field Microscopy

Question 27

uses UV light, under which only fluorescent molecules are visible

Answer 27

Fluorescence Microscopy

Question 28

uses the difference in refractive index of various natural cell and tissue components to produce an image without staining

Answer 28

Phase-Contrast Microscopy

Question 29

involves scanning the specimen at successive focal planes with a focused light beam, often from a laser, and produces a 3D reconstruction from the imgs

Answer 29

Confocal Microscopy