Intro Flashcards
study of tissues of the body and how these tissues arrange to constitute organs
Histology
this is also termed as microscopic anatomy
histology
this is also termed as microscopic anatomy
histology
What do you call a group of cells with interrelated function?
Tissue
What are the two components of a tissue?
Cells and ECM (Extracellular Matrix)
What are the two components of a tissue?
Cells and ECM (Extracellular Matrix)
a process wherein tissue structure is preserved, autolysis is prevented, and pathogenic microorganisms are killed
autolysis = cell death caused by enzymes
fixation
the most common fixative is?
formalin
If the tissue is calcified, this is the step before dehydration
“unhardening”
Decalcification
this is the step wherein tissue is bathed successively through a series of increased concentrations of alcohol sol’ns, ending in 100%, which **removes all water **
dehydration
the step wherein alcohol from tissue is removed in organic solvents in which alcohol and paraffin are miscible
clearing
process wherein the paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden
embedding
this is where the paraffin block is trimmed to expose the tissue for thin slicing
trimming
the chemicals used for clearing
xylene and toluene
the most commonly used stain
H&E - Hematoxylin and Eosin
this stains the DNA in the nucleus, RNA-rich portions of the cytoplasm, and produces a dark blue or purple color
Hematoxylin
stains the other cytoplasmic structures pink
Eosin
what do you call the negative parts in a tissue that adheres to a basic dye
Basophilic
Adheres to a basic dye = basic lover
what do you call the positive parts in a tissue that adheres to an acidic dye
Acidophilic
Acid lovers
which among the H&E is acidic? is basic?
Hematoxylin - basic
Eosin - acidic
Thus:
Hematoxylin = for negative parts
Eosin = for positive parts
negative parts = basophilic
positive parts = acidophilic
Feulgen test
DNA
Periodic Acid Schiff (PAS)
Carbohydrates
Sudan Black
Lipids
Silver Stain
Reticular Fibers
Glycolysis without oxygen results to the production of?
Lactic Acid
the method most commonly used in microscopy; uses ordinary light and the colors are imparted by tissue staining
Bright-Field Microscopy
uses UV light, under which only fluorescent molecules are visible
Fluorescence Microscopy
uses the difference in refractive index of various natural cell and tissue components to produce an image without staining
Phase-Contrast Microscopy
involves scanning the specimen at successive focal planes with a focused light beam, often from a laser, and produces a 3D reconstruction from the imgs
Confocal Microscopy
