Instrumentation in organic chemistry Flashcards
What is chromatography?
Chromatography is a separation technique is which a moblie phase carries a mixture of components through a stationary phase
The componests in the mixtire are selectively adbsorbed onto the stationary phase and pass through at different times
What is a mixture
substance consisting of two or more substances migled together but not chemically combined
Name the thee types of chromotography
paper chromatography/
Gas chromatography/
High performance liquid chromotography (HPLC)
Describe the procedure
Paper chromatography
Using a dropper, place a drop of ink or indicator 2cm up from the bottom of the chromotography paper on a pencil line drawn.
Place the chromatography paper into a beaker with 1cm of suitable eluent
leave for 1 hour
Describe the result
Paper chromatography
As the eluent moblie phase moves up the chromotography paper, each component colour in the ink is selectively adsorbed and appears in the chromotography paper stationary at differnt times
what is the mobile phase and the stationary phase
Paper chromatography
Moblie phase : liquid eluent/ solvent
Stationary phase: chromatography paper
Name a suitable solvent for a soluable mixture and an insoluble solvent
Paper chromatography
Water
(Insoluble - methanol)
Why is the line drawn on to the chromotograpy in pencil
Paper chromatography
avoids contamination with other types of ink
Where should the pancil line be drawn on the chromotography paper
Paper chromatography
2cm up the chromotopraphy paper