Instrumentation in organic chemistry Flashcards

1
Q

What is chromatography?

A

Chromatography is a separation technique is which a moblie phase carries a mixture of components through a stationary phase
The componests in the mixtire are selectively adbsorbed onto the stationary phase and pass through at different times

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2
Q

What is a mixture

A

substance consisting of two or more substances migled together but not chemically combined

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3
Q

Name the thee types of chromotography

A

paper chromatography/
Gas chromatography/
High performance liquid chromotography (HPLC)

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4
Q

Describe the procedure

Paper chromatography

A

Using a dropper, place a drop of ink or indicator 2cm up from the bottom of the chromotography paper on a pencil line drawn.
Place the chromatography paper into a beaker with 1cm of suitable eluent
leave for 1 hour

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5
Q

Describe the result

Paper chromatography

A

As the eluent moblie phase moves up the chromotography paper, each component colour in the ink is selectively adsorbed and appears in the chromotography paper stationary at differnt times

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6
Q

what is the mobile phase and the stationary phase

Paper chromatography

A

Moblie phase : liquid eluent/ solvent
Stationary phase: chromatography paper

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7
Q

Name a suitable solvent for a soluable mixture and an insoluble solvent

Paper chromatography

A

Water
(Insoluble - methanol)

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8
Q

Why is the line drawn on to the chromotograpy in pencil

Paper chromatography

A

avoids contamination with other types of ink

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9
Q

Where should the pancil line be drawn on the chromotography paper

Paper chromatography

A

2cm up the chromotopraphy paper

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