Inhibitors of Cell Wall synthesis Flashcards
Cell wall synthesis inhibitors
B-lactams abs - Penicillins - Cephalosporins - Carbapenems - Monobactams Vancomycin Daptomycin Bacitracin Fosfomycin
require actively proliferating bactera (cell wall synthesis must be occuring)
Penicillin-binding proteins (PBP)-
bacterial enzymes involved in cell wall synthesis. Target site for B-lactam antibiotics.
Penicillin
MOA?
what king of organisms is it inactive against?
- Inhibits last step of peptidoglycan synthesis through binding to PBPs.
- Penicillins also activate autolysin (bacterial enzyme) to initiate cell death by lysis and inhibiton of cell wall assembly. - Inactive against organisms w/out peptidoglycan cell wall. eg. mycoplasma/ protozoa/ fungi/ viruses
Natural penicillins: Penicillin G
- what is it active against?
- What is t susceptible to?
Benzylpenicillin
- active against:
- Most gram positive cocci (NOT STAPH)
- Gram-postive rods (eg. Listeria/ C- perfringes)
- Gram negative cocci (eg. Neisseria)
- Most anaerobes (NOT BACTERIODES) - Susceptible to inactivation by B-lactamases
What is the DOC for:
Syphilis
Strep infections (especially in prevention of rheumatic fever)
Susceptible pneumococci
Penicillin G
- Benzathine penicillin G for syphilis and Rheumatic fever prophylaxis.
Repository Penicillins are used for:
Mode of transmission is through:
Prolonging Penicillin G by increasing t1/2.
- Penicllin G procain
- Penicillin G Benzathine
Intramuscular not IV (risk of procaine toxicity)
- seldom used (increased resistance
Natural penicillins: Penicillin V
Administration?
DOC?
less sensitive ti Gram -ve bacteria than G
More acid stable (Can be given orally)
DOC for Strep throat
- employed orally for mild-moderate infections eg. pharyngitis/ tonsilits/ skin infections (caused by strep throat)
‘Antistaphylococcal’ penicillins
special trait:
First line tx for:
Methilcillin Nafcillin (Naf for staph) Oxacillin Dicloxacillin - B-lactamase resistant - Inactive against MRSA
First line for staphylococci endocarditis in patients w/out artificial heart valves.
Extended-spectrum penicllins
Ampicillin
Amoxicllin
Similar to penicillin G (+ gram negative activity)
susceptible to B-lactamases
activity enhanced w/ B-lactamase inhibitor
Amoxicillin special because
Highest oral bioavailabilty than other penicillins
also common ab prescribed for children an pregnancy
Ampicillin and Amoxicillin clinical appllications
Acute otitis media
streptococcal pharyngitis
pneumonia
skin infections
UTIs
- widely used to treat upper respiratory infections (H. Influenzae and S. pneumoniae)
- Prophylaxis against endocarditis during dental and respiratory tract procedures.
Ampicillin + aminoglycoside are used to treat
Enterococci
Listerial infections
Antipseudomonal Penicillins
Active against what type of organisms?
Carbenicillin
Ticarcillin (+ clavulinate B-lactamase inhibitor)
Piperacillin (+ Tazobactam)
Effective against many gram -ve and +ve bacilli
Often combined w/ B-lactamase inhibitor
Active against P. aeruginosa
Carbenicillin
Ticarcillin
Piperacillin
Clinical applications:
- Pseudomonas aeruginosa treatment
- Main clinical use= as an injectable tx. of Gram -ve’s
Treatment of moderate to severe infections of susceptible organisms (eg. uncomplicated and complicated skin/ gynecologic and intra-abdominal infections/ febrile neutropenia)
Penicillin + aminoglycoside
DOC for?
Synergistic
Penicillins facilitate movement of aminoglycosides through cell wall
- Never place in the same infusion fluid (form inactive complex)
DOC: Effective emperic tx. for infective endocarditis
Penicillin PK
- Oral absorption?
- Nafcillin oral absorption?
distribution?
Impaired by food
t1/2=30-60 mins (except for repository penicillins)
Nafcillin = erratic (not suitable for oral admin)
Penicillin PK
distributiontion?
Excretion?
All achieve therapeutic levels in pleural pericardial pertioneal synovial fluids and urine
Naficillin ampicillin and piperacillin achieve high levels in bile
levels in prostate and eye = insufficient
CSF penetration = poor (except in meningitis)
Excreted mostly in Kidney
Nafcillin = exception as primarily excreted in bile*
Penicillin AE
Hypersensitivity*
- Penicillic acid = major antigenic determinant
Gi disturbances (eg. diarrhea)*
Pseudomembranous colitis (ampicillin)*
Maculopapular rash (ampicilln/ amoxicillin)*
Interstitial nehritis (methicillin)
Neurotoxicity (epileptics at risk)
Hematologic toxicities (ticarcillin)
Neutropenia (naficillin)
Hepatitis (oxacillin)
Secondary infections (eg. Vaginal candidiasis)
B-lactamase inhibitors
MOA?
Clavulanic Acid
Sulbactam
Tazobactam
MOA: Bind to and inactivate most B-lactamsases
- do not have significant anti bacterial activity.
Cepahalosporins are considered inactive against?
"CAMELL" Enterococci Listeria Legionella Chlamydia Mycoplasma Acinebacter
4th Gen. cephalosporins are similar to which other generations?
What similarities do they both share?
Similar to 1st gen. against gram +ve cocci and are also active against most gram -ve bacilli.
5th Gen. cephalosporins are similar to which other generations?
Whats unique about 5th gen?
Have similar spectrum to the 3rd gen.
They are unique in that they have activity against MRSA