Industrial microbio Flashcards
primary metabolites are produced during the exponential phase - example?
alcohol
secondary metabolites are produced at the end of growth, near the _____
stationary phase
antibiotics are
secondary metabolites
example of industrial microbio products
antibiotics enzymes food additives chemicals alcohol
Fermentation refers to
any large scale microbial process (anaerobic or aerobic)
wild type strains are normally not suitable, instead use?
mutant strains that overproduce the desired metabolites. deregulated mutant leads to large scale production of the compound
genetical modified organisms to ___
overproduce desired compound. can control expression
- examples are human insulin and rennet
production of samotpotropin
- human growth factor
- defeciency causes dwarfism
Insuline (hormone) production in body
produced by beta cells of pancreas. insulin injection needed for type 1 diabetes
what was the first human protein to be produced by bacteria?
insulin
how do bacteria produce insulin?
put human insulin gene into Recombinate bacteria–> fermentation–> isolate insulin –> put into pills
Genetically engineered pant with roundup, how?
Glyphosate (roundup) kills plants by inhibiting amino acid synthesis. Take roundup resistant gene from bacteria and put into plant seed. now plants are resistant. by all other weeds will die
resistance to insects
put gene that encode insect toxin into plant