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SEM2 - I/S > Immunology > Flashcards

Immunology Flashcards

1
Q

1) RID

2) Ouchterlony double diffusion

A

Mancini/endpoint method

Fahey&mckelvey/kinetic

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2
Q

Both ag-ab diffuse independently thru a semi-solid medium
Serologic identity =
Non-identity =
Partial identity =

A

Ouchterlony DD
smooth curve
crossed line
spur-formation ; ab precip. ag w/ common determinant

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3
Q

Precipitation by electrophoretic techniques

A
  1. rocket immunoelectrophoresis
  2. countercurrent immunoelectrophoresis
  3. immunofixation electrophoresis
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4
Q

Rocket

A

RID + EP

Height/apex = Ag conc

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5
Q

Countercurrent EP
Source of Ag
Sign of abnormality

A

Px serum wc is EPd in separate protein fractions

Change in shapre, loc, and intensity of precipitin arc

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6
Q

Phases of agglu

A

Sensi

Lattice form

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7
Q

enhancement oflattice f

A

LISS, PEG, enz, pH, adjusting temp

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8
Q

Types of agglu

A 
Direct
Passive
Reverse Passive
Coagglu
Agglu Inhibition
Antiglobulin-mediated agglu
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9
Q

Direct agglu

Ex.

A

Ex. Hemagglu = Ag is found on RBCs

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10
Q

Passive agglu

Ex.

A

Ex. ASO latex agglutination test

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11
Q

Ag on surface of particle

A

Direct agglu

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12
Q

Ag is attached to the carrier particle

A

Passive agglu

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13
Q

Agglu occurs if px Ab is present

A

Passive agglu

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14
Q

Ab is attached to the c.part

A

Reverse passive agglu

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15
Q

Agglu occurs if px ag is present

A

Reverse passive agglu

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16
Q

Rev-Pass agglu

ex.

A

CRP LAT

17
Q

Uses bacte as inert particles to wc ab are attached

A

coagglu

18
Q

Positive result is lack of agglu

A

agglu inhib

19
Q

agglu inhi ex.

A 
B HCG
Hemagglu inhi (influenza, rubella, measles)
20
Q

Antiglobulin-mediated agglu ex.

A

Coomb’s
DAT
IAT

21
Q

DAT

IAT

A

Px RBCs + AHG = agglu (+)

sensi thru incubation -> wash 2-3x -> AHG

22
Q

analyte measured competes w/ a radiolabeled analyte for a limited no. of binding sites

A

compet RIA

23
Q

amt of label is inv propo to the amt of px ag

A

compet RIA

24
Q

amt of bound labelled Ab is directly propo to the amt of px analyte

A

non compet IRMA

25
Q

labeled immunoassays

A

RIA
EIA
Fluorescent immunoassay

26
Q

EIA

A

horse radish perox
g6pd
glucose oxidase
b-galactosidase

27
Q

based on princip of RIA

A

compet ELISA

28
Q

enzyme act is indirectly prop to the conc of analyte

A

compet ELISA

29
Q

indirect ELISA

A

non compet ELISA

30
Q

enzyme-labeled rgt does not participate to the initial ag-ab rxn

A

non compet ELISA

31
Q

amt of enzyme label is direct propo to the con of analy

A

non compet elisa

32
Q

enzyme act is directly propo to the ag conc

A

sandwich/capture EIA

33
Q

direct immunof assay

A

unknown ag + FITC/Tritc labeled ab = + fluor

34
Q

indirect IF

A

known ag + unknown ab + fitc/titc labeled AHG = + fluor

SEM2 - I/S (7 decks)

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