Immunology Flashcards
soluble antigen + antibody
crosslinking causes positive
precipitation assay
insoluble antigen + antibody
clumping causes positive
agglutination assay
2 types of agglutination assay
- direct: antigen is insoluble
- indirect: soluble antigen is attached to a particle, like a latex bead
explain double diffusion method
AKA immunodiffusion
antigen and antibody migrate toward one another in gel
lines meet perfectly: identity, +
one line is longer: partial identity, =
lines cross: nonidentity, =
prozone
antibody excess
false =
postzone
antigen excess
false =
positive vs negative on complement fixation assay
positive: no hemolysis; C’ is bound to patient’s Ag-Ab complexes
negative: hemolysis; C’ is not bound
IgM anti-IgG
rheumatoid factor
disguises an IgG as an IgM
rheumatoid factor
high RF titer
SLE
RA
low RF titer
syphilis
hepatitis
elderly
explain nephelometry
precipitation test read by spectrophotometer
4 stages of syphilis
primary: lesion
secondary: rash
latent: no sx
tertiary: systemic involement; neurosyphilis
nontreponemal tests
detect what?
RPR & VDRL
screening
detects anti-cardiolipin reagin Ab
treponemal tests
IFA, TPA
confirmatory
organism is the Ag
flocculation test
RPR
anti-cardiolipin
positive in syphilis patients
when are RPR and VDRL positive?
1° and 2°syphilis
when are treponemal tests positive?
any stage of syphilis
looks for neurosyphilis in the secondary stage
done on CSF
VDRL
false + RPR test
malaria
mono
SLE
other autoimmune
cells used to test for anti-I/i
adult and cord O cells, washed and kept warm
neutralization test that may help dx Strep group A or sequelae
ASO
(streptolysin O)
Coomassie stain
CSF protein electrophoresis
prealbumin band
shows up in CSF
CSF index & normal
index = (CSF IgG/CSF alb)/(serum IgG/serum alb)
normal —<0.66
elevated CSF index
MS
syphilis, other diseases causing CNS IgG production
uses specific anti-sera to ID monoclonal & BJ proteins
immunofixation
Hep2
ANA substrate
ANA homogeneous pattern
SLE
reflex to nDNA
nDNA +
organism
Crithidia lucillae
kinetoplast fluorescence
SLE
anti-ssDNA +
drug-induced lupus
ANA rim pattern
SLE
ANA speckled pattern
reflex to ENA
ENA Smith +
SLE
ENA RNP +
SLE, MCTD
ENA SSA/SSB +
Sjogren’s syndrome
ANA centromere pattern
CREST syndrome
ANA nucleolar pattern
PSS or SLE
MSK
renal tubules & parietal cells
AMA
primary biliary cirrhosis
MSK
stomach muscle & blood vessel walls
ASMA
chronic active hepatitis
MSK
Parietal cells
APCA
pernicious anemia
C-ANCA and P-ANCA
Wegener’s granulomatosis
systemic vasculitis
ACh receptor antibody
myasthenia gravis
thyroglobulin Ab
Hashimoto’s
purpose of direct IFA
look for immune complexes in patient tissue
HBV decreased infectious state
HBeAb +
RIBA method
HCV IgG
used most frequently to screen for HIV
HIV1/2Ab + p24 Ag test
2/3 antigens that need to be + on Western blot for HIV confirmation
p24
gp41
gp120/160
earliest HIV marker
HIV p24 test
used to monitor HIV patients
- HIV RNA - viral load
- HIV genotyping - mutations
- CD4/CD8 ratio
CD4 count indicating AIDS
<400
HAART
highly active antiretroviral therapy
for HIV
low MW particle requiring a carrier for immunogenicity
hapten
most reliable test for Lyme
Western blot
first Ab on surface of new B-cells
IgD
CD38
plasma cell marker
causes class switching
CD40 (B) + CD40 ligand (T)
need presentation on MHC II
CD4
need presentation on MHC I
CD8
deficiency of C1 esterase inhibitor
hereditary angioedema (HANE)
measures complement activity, not concentration
CH50 test
C’ proteins are ——– labile
heat
freeze serum immediately for C’ testing
IgE hypersensitivity
allergies
histamine
type I
complement hypersensitivity
transfusion rxn
type II
ag-ab complex hypersensitivity
Arthus rxn
Type III
delayed hypersensitivity
T-cell mediated
type IV
IgG deficiency
no mature B-cells
Bruton’s agammaglobulinemia
no/few T-cells due to failure of thymus to develop
DiGeorge syndrome
low IgM
males
thrombocytopenia, small plts
Wiskott-Aldrich syndrome
low level of lysosomal enzymes
partial albinism
giant cytoplasmic inclusions
Chediak-Higashi
G6PD deficiency
MPO and ALP ↓ in neutrophils
chronic granulomatous disease