Immunological Tests/Techniques

Question 1

Radioallergosorbent test (RAST)

Answer 1

blood test used to determine the substances a subject is allergic to

Question 2

ELISA

Answer 2

measures antigen concentrations using enzyme-conjugated Ab

Question 3

Immunoblotting

Answer 3

protein electrophoresis/transfer to membrane/antibody detection

Question 4

Hemagglutination inhibition

Answer 4

to quantitate serum antibody to a specific virus (ex flu)

Question 5

Mixed lymphocyte reaction

Answer 5

to measure allogeneic T cell response

Question 6

Southern blotting

Answer 6

DNA electrophoresis/transfer to membrane/hybridization

Question 7

Northern blotting

Answer 7

RNA electrophoresis/transfer to membrane/hybridization

Question 8

Immunofluorescent staining

Answer 8

labeling with fluorescent probes/microscopy

Question 9

Immunoelectrophoresis

Answer 9

performed to detect immunoglobulin classes of complement proteins in plasma

Question 10

Nitroblue tetrazolium dye test

Answer 10

test of phagocyte respiratory burst

Question 11

Flow cytometry for detection of cell-bound antigens by fluorescent antibodies

Answer 11

for detection of cell-bound antigens by fluorescent antibodies