Immunohematology Final Lab Practical (Labs 7-14) Flashcards
At which phase of testing do most clinically significant allo-antibodies react? (2)
37C (Incubation) & AHG
Why is it important to match the lot number of panel cells used in the antigram?
To appropriately identify the patient’s antibody. Each antigram has a different antigen expression on the cells. Using the wrong antigram could identify the wrong antibody.
If polyspecific AHG is used for a panel, what would cause an IgM to react?
Complement (C3)
Which antibody is most likely?
C
Which antibody(ies) cannot be ruled out?
Kell
Why is cell #3 weaker than the others?
Dosage - heterozygous for C/c
Which of the following additional cells need to be run?:
a. C=, K+ cell
b. C+, K+ cell
c. C=, K+ cell
d. C+, K= cell
a. C=, K+ cell
Which antibody or antibodies cannot be ruled out or half-crossed? (7)
-C
-e
-S
-P1
-Lewis a
-K
-Fy b
Does the reactivity fit any single antibody?
Yes, little e.
How would you eliminate the antibodies not crossed off? (select all that apply):
a. Phenotype the patient
b. Run select cells
c. Neutralization
d. DTT
a. Phenotype the patient
b. Run select cells
If a patient’s antibody screen is positive, how can the identity of the antibody be determined?:
a. Perform a panel
b. Perform an elution
c. Perform a weak D test
d. Request a redraw
a. Perform a panel
All of the following will result in a false negative IAT except:
a. Failure to add patient plasma
b. undercentrifugation
c. Correctly following the IAT procedure, adding patient plasma, commercial screening cells, and AHG
d. Failure to add AHG
c. Correctly following the IAT procedure, adding patient plasma, commercial screening cells, and AHG
Failure to add patient plasma, undercentrifugation, and failure to add AHG will cause what type of result for IAT testing?
False negative
The following is used to denote what type of reaction on a data sheet?
Microscopic positive
Determine the patient’s ABO given the following reactions:
A2B positive
Determine the patient’s ABO given the following reactions:
A2 with anti-A1, Rh invalid due to positive reacting weak D control
Determine the patient’s ABO given the following reactions:
A1 positive
Based on the following IAT results, what type of antibody is most likely present?
(warm/cold, auto/allo, IgG/IgM)
Warm auto antibody
Based on the following IAT results, what type of antibody is most likely present?
(warm/cold, auto/allo, IgG/IgM)
Cold auto antibody
Based on the following IAT results, what type of antibody is most likely present? (single/multiple, auto/allo, IgG/IgM, warm/cold)
Multiple IgG antibodies
Based on the following IAT results, what type of antibody is most likely present? (single/multiple, auto/allo, IgG/IgM, warm/cold)
Single allo IgG antibody
Interpret the reaction:
B with anti-I
Interpret the reaction:
A2 with anti-A1
Interpret the reaction:
A1 with anti-H
Interpret the reaction:
A2B
Interpret the reaction:
B with cold alloantibody
Interpret the reaction:
O with anti-i
Interpret the reaction:
A1 with anti-IH
A1 with anti-I
AB with anti-i
O with alloantibody
Which antibody is most likely?
Lewis A
From the panel, which antibody(s) cannot be ruled out?
All are ruled out
What additional method could be used to confirm antibody identification?
Neutralization
What is the most likely phenotype of Jane Doe?
LeA=, LeB=
(have to be negative for both to create an antibody)
Which antibody(s) is most likely present?
Kell
What percent of the white population would be compatible with this antibody?
a. 2%
b. 9%
c. 98%
d. 91%
d. 91%
What percent of the white population would be compatible with this antibody and be the same ABO/Rh type?
a. 44%
b. 35%
c. 6%
d. 68%
b. 35%
-Type O: 45%
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