Imaging Flashcards
What preserves tissue samples?
Formalin
What substance helps tissue slicing?
Paraffin
Name the most common stains
Haematoxylin and Eosin
How cold air frozen sections?
-20 to -30
What cuts tissue samples really thin?
Microtome
When would a frozen section be used and what’s the major drawbacks?
Quick histology required, but poor technical quality
What’s the differential diagnosis of gout?
Pseudogout
What can be used to confirm gout?
Polarised light microscopey
Why is joint fluid imaging better than measuring serum urea?
Accute gout means a lot of the urea has precipitated in to the join already so blood plasma levels are low
What technique is useful for differentiating cancer cell types?
Fluorescent microscopey
You need live tissue imaging, what test will you use?
Confocal microscopey - pinhole in front of observer to reduce blur
What clinical imaging technique uses confocal microscopy techniques?
CAT scans
Immunohistochemisrty includes two techniques- compare them.
Indirect immunohistochemistry an antibody binds to the target. Another labelled antibody attaches to this and produces a coloured chemical when it binds.
Immune fluorescence involves the antibody targeting the antigen is labelled itself
What can autoradiography do?
Inject radioactive marker and then take Histological sample.
Iodine 131 is used in autoradiography for ?
The thyroid
