ii. Bacterial Shape And Size Flashcards
Largest bacteria
Thiomargarita namibensis
Smallest bacteria
Mycoplasma sp
Largest medically important bacteria
Borrelia burgodorferi (lyme disease)
Only bacteria without a peptidoglycan cell wall
Mycoplasma
Peptidoglycans are crosslinked by
Transpeptidase
Targeted by lysozymes
B 1-4 linkage bn GlcNac and MurNac
Unique to gram positive organisms
Teichoic acid
Unique to gram negative organisms
Endotoxin/LPS
Only gram positive with an endotoxin
Listeria monocytogenes
Perisplasmic space found in?
Gram negatir
Gram staining procedure
Crystal violet, iodine, acetone, safranin
Primary stain
Crystal violet
Mordant
Iodine
Decolorizing agent
Acetone
Counterstain
Safranin
Mycobacteria stain
Acid fast stain
Spirochetes altenative approach to staining
Darkfield microscopy
Mycoplasma altenative approach to staining
Serology
Legionella spp altenative approach to staining
Silver stain
Chlamydia altenative approach to staining
Inclusion bodies
Rickettsiae altenative approach to staining
Giemsa/ tissue stain
Essential components of bacteria
Cell wall, cytoplasmic membrane, ribosome, nucleoid, mesosome, periplasm
Contains many hydrolytic enzymes including B lactamases
Periplasm
Non essential components of bacteria
Capsule, pilus/ fimbriae, glycocalyx, flagellum, spore, plasmid, granule
Protects against phagocytosis
Capsule
For attachment or conjugation
Pili, fimbriae
Mediates adherence to surfaces
Glycocalyx
Genes for antibtiotic resistance and toxins
Plasmid
Site of nutrients in cytoplasm
Granule
Only bacterial capsule that is composed of polypeptide of D- glutamate
Bacillus anthracis
Example of spore forming gram + rods
Bacillus and clostridium
Toxic proteins produced by certain bacteria that are lethal for other bacteria
Bacteriocins