Humane endpoints, surgery, euthenasia

Question 1

What is an experimental endpoint?

Answer 1

predefined endpoint of experiment when alle necessary data has been collected.

Question 2

What is a humane endpoint?

Answer 2

The earliest point the experiment can or should be terminated. Purpose of eliminating suffering. Might be before the experimental endpoint.

Question 3

How do we define and detect the humane endpoint?

Answer 3

1) take into accout the severity of the procedure (mild, moderate etc)
2) Have a score sheet with relevant parameters which are monitored (behaviour, appearance, clinical signs - both qualitative and quantitive) - should be specific to strain, species.

Question 4

What is a subclinical infection?

Answer 4

Infection without symptoms that can be seen clinically but might have influence on experimental data.

Question 5

What´s important when performing surgery?

Answer 5

1) working aseptically = no transfer of microorganisms.

2) plan procedure beforehand

Question 6

How to work sterile:

Answer 6

1) sterile only touch sterile
2) unsterila only touch unsterile
3) sterile doesn´t touch unsterile

Question 7

checklist for surgery:

Answer 7

1) choice of animals
2) evaluation of animal health
3) facilities
4) surgical instruments - cleaning and aseptic
5) number of assistants
6) prepare animal for surgery - fasting, shaving, cleaning, acclimitisation
7) management of animal
8) aneasthesia protocol
9) adequate practise
10) record keeping

Question 8

How can i confirm death?

Answer 8

1) confirmation of permanent cessation of the brain
2) destruction of the brain
3) dislocation of the neck
4) exsangunation
5) rigor mortis

Question 9

which euthenasia technique is allowed in all species?

Answer 9

aneasthetic overdose