HTP Flashcards
Which of the following statements is true about heat fixation
a. It is used routinely
b. It is used in conjunction with frozen section processing
c. Either d. Neither
b. It is used in conjunction with frozen section processing
Swelling of a glacial acetic treated tissue is prevented by the addition of one the following fixatives?
a. Mercuric chloride
b. Alcohol
c. Chromates
d. All of the above
All of the above
Fixation of the tissue in liquefied nitrogen and followed by dehydration in acetone is called as which of the following processes?
a. Freeze-drying
b. Lyophilization
c. Freeze-substition
d. Quenching
Freeze-substition
Which fixative is considered as the secondary fixative for a formalin-fixed specimen
a. Alcoholic formalin
b. Helly’s fluid
c. Zenker’s fluid d. 100% alcohol
Helly’s fluid
Which one of the following fixatives is not recommended if H&E staining of the tissue section will be done
a. Alcohol
b. Osmic acid
c. Chromic acid d. Acetone
Osmic acid
- Dehydration is necessary step in which of the following tissues?
a. Frozen sections
b. Specimens for enzyme study c. Routine specimen
d. A and B
Routine specimen
Dehydration in acetone requires which of the following conditions
a. Use of ascending concentration
b. Use of descending concentration
c. Either
d. Neither
Neither
Which of the following tissues is best infiltrated using the vacuum oven
a. Urgent biopsies
b. Kidney slices
c. Lung tissue
d. Bone fragment
Lung tissue
- Which is considered the best method for the infiltration of fatty tissue and those for enzyme study
a. Paraffin method
b. Celloidin method
c. Carbowax method
d. Agar method
Carbowax method
Which of the following makes paraffin “softer” and more resilient
a. Carbowax
b. Beeswax
c. Collodion
d.BandC
d.B and C
Infiltration in paraplast requires the use of which of the following
a. 100% solution
b. A 1:1 paraffin plastic mixture
c. Either d. Neither
A 1:1 paraffin plastic mixture
Which is correct for the infiltration of resin
a. A 1:1 Resin-propylene oxide; Resin I; Resin II
b. Propylene oxide; 1:2 resin propylene oxide; Resin I
c. Glutaraldehyde; osmic acid; Resin I; Resin II; Resin III d. Resin I; Resin II; Resin III
a. A 1:1 Resin-propylene oxide; Resin I; Resin II
The embedding molds include all of the following, except:
a. Tissue-tek
b. Tissue cassette
c. Shallow pan with tight fitting cover d. Leukhart L pieces
Tissue cassette
When embedding, one of the following is not observed
a. Push the tissue to the center of the mold
b. Orient the tissue properly
c. Avoid introduction of bubbles
d. Immediately place the block on ice/cold water
Immediately place the block on ice/cold water
- Which of the following is the main reason for embedding
a. To obtain tissue ribbon
b. For better microscopic examination c. To facilitate handling of tissue
d. Any of the above
To facilitate handling of tissue
Embedding in celloidin is complete if one of the following event is present
a. The block is hard when pressed
b. When the block is covered with a white powdery state
c. When celloidin no longer sticks on one’s finger
d. None of the above
When celloidin no longer sticks on one’s finger
Celloidin embedding is done correctly using which method below
a. Celloidin is poured about 1⁄4 full, then carefully place in the mold and add more up to the brim
b. Celloidin is added to the tissue and the solvent is evaporated until it dries up
c.The embedding mold is opened 30 minutes daily for one week to
evaporate the solvent until the celloidin gels
d. The solvent is added, the container is covered tightly and allowed to come contact with the tissue for atleast one week
The embedding mold is opened 30 minutes daily for one week to
evaporate the solvent until the celloidin gels
Embedding resin uses which of the following mold?
a. BEAM capsule b. Egg capsule
c. BEEM capsule d. Space capsule
BEEM capsule
Casting of the soft tissue in gelatin is done at this temperature
a. 0-4°C
b. 22-25°C c. 37°C
d. 56°C
a. 0-4°C
- Infiltration in carbowax uses one of the ff. temperatures
a. °2C -5°C above the MP b. 2°C -5°C below the MP c. 56°C -58°C
d. 50°C -55°C
c. 56°C -58°C
One of the ff. is added to preserve the paraffin wax used for embedding
a. 1% hypochlorite
b. 1% phenol
c. 1% thymol
d. None
None
Infiltration is also known as:
a. Casting
b. Impregnation
c. Embedding d. None
Impregnation
What is the melting point of paraffin recommended for the infiltration of breast tissue
a. 56°C -62°C
b. 45°C -58°C
c. 48°C -58°C
d. 50°C -55°C
c. 48°C -58°C
Infiltration and embedding in epoxy resin requires the use of which of the following
a. Dodecenyl succinic anhydride
b. DMP-30
c. Both
d. None
c. Both
- Celloidin processing uses which clearing agent
a. Alcohol-ether
b. Propylene oxide
c. Xylene
d. Hydroxyl methoxide
Alcohol-ether
Which method provides the fastest means of paraffin infiltration
a. Autotechnicon method
b. Cryostat method
c. Vacuum oven method
d. All
Vacuum oven method
Which of the following infiltration methods avoid thermal destruction of tissue
a. Carbowax
b. Celloidin
c. Esterwax
d. Gum Arabic
Celloidin
Which of the following statement is true regarding ester wax
a. Harder than paraffin
b. Has a lower melting point c. Use for tough speciments d. All of the above
All of the above
Which of the following celloidin concentrations is used for infiltration
a. 2-14% solutions
b. 14% solutions
c. 20% solutions
d. 100% concentration
a. 2-14% solutions
- If you see a person being electrocuted, what should you first remember and do?
A. Do not touch them
B. Turn off the power
C. Pry them away from the contact point D. Call for help
Do not touch them
