Bacteria Retake Flashcards
Which of the following urine samples is NOT acceptable for bacterial culture?
a. Straight Catheterized Urine
b. Routinely Avoided Urine
c. Midstream Clean Catch Urine
d. Suprapubic Aspiration
Routinely avoided urine
What is the proper concentration of the anticoagulant used for blood culture?
a. 0.025% Sodium Polyethanol Sulfonate
b. 0.25% Sodium Polyanathol Sulfonate
c. 0.025% Sodium Polyanaethanol Sulfonate
d. 0.25% Sodium Polyanaethanol Sulfonate
0.025% Sodium Polyanaethanol Sulfonate
For at least how many millilitres of blood for culture is collected from a 52 year old immunocompromised patient?
a. 10 mL per set of aerobic & anaerobic bottles
b. 20 mL per set of aerobic & anaerobic bottles-
c. None of the choices are correct
d. Both of the choices are correct
20 ml
What swab is used for collection of cervical and vaginal secretions?
a. Alginate b. Cotton c. Rayon d. None
Rayon
Specimen collection of CSF
a. Within 1 hour
b. Within 2 hours
c. WIthin 30 mins
d. Within 15 mins
Within 15 mins
The following buffered sodium media can contain microorganism at 72 hours:
a. Sodium thiosulfocollate
b. Sodium thioglysulfate
c. Sodium thioglycollate
d. Sodium thiosulfate
Sodium Thioglycollate
Which of the following terms are used to describe microorganisms that grow under reduced 0, 10% & increased CO, 10%?
a. Aerobes
b. Anaerobes
c. Microaerophilic d. Capnophiles
Microaerophilic
Bsc class used for radioisotopes and 30% of air is exhausted and 70% is recirculated
a. Class I
b. Class II A1
c. Class II A2
d. Class III
Class II A2
An MLS intern was tasked to perform gram staining. Upon checking, the intern noticed that the acid alcohol decolorizer is in blue color. What should be done by the intern
a. Report the incident
b. Discard the reagent and
report the incident
c. Repeat staining
d. The intern done it logically
and correct
Discard the reagent and report the incident
Components of 100mL Kinyouns Carbolfuchsin
a. 4 g basic fuchsin, 8 g phenol, 20 mL 95% alcohol, 100 mL distilled water
b. 8 g basic fuchsin, 4 g phenol, 100 mL 95% alcohol, 20 mL distilled water
c. 2 g basic fuchsin, 6 g phenol, 20 mL 95% alcohol, 100 mL distilled water
d. 6 g basic fuchsin, 2 g phenol, 100 mL 95% alcohol, 20 mL distilled water
4g basic fuchsin, 8g phenol, 20ml 95% alcohol
All of the following bacteria species below are either distinctly acid fast or partially acid fast. Which of the following is not an acid fast bacteria species?
I.Nocardia spp.
II.Rhodococcus spp.
III.Cryptosporidium spp.
IV.Clostridium spp.
a. I only
b. II and III
c. IV only
d. I and IV
IV only
Which of the following statements best supports the action of the detergent in Kinyouns method
a. Detergent in the solution enhances the stain’s color
b. It acts as a decontaminant for
other pathogens that may be
present in the specimen
c. Detergent is used to force the
carbolfuchsin into the
mycobacterial cell wall
d. Detergent acts as a mordant
in acid-fast staining
Detergent is used to force the carbolfuchsin into the mycobacterial cell wall
How would you describe acid-fast bacteria under the brightfield microscope using your Ziehl-Neelsen stain?
a. -Blue microorganisms on a red background
b. -Red microorganism on a red background
c. -Red microorganisms on a blue background
d. -Blue microorganisms on a blue background
Red microorganisms on a blue background
What is the function of the mycolic acid in acid fast bacteria?
a. It renders the bacteria resistant to harsh chemicals
b. It is an exotoxin that helps it multiply once in the host’s body
c. Mycolic acid is what helps the medical technologist determine if it is an acid fast bacteria
d. All statements are true
It renders the bacteria resistant to harsh chemicals
Which among the ff is not a common dermatophyte
a. Tinea barbae
b. Tricophyton rubrum
c. Tinea cruris
d. Aspergillus niger
Aspergillus niger
Which fungi is found in nails and causes onychomycosis
a. Tinea capitis
b. Tinea corporis
c. Tinea unguium
d. Trichophyton mentagrophytes
Tinea unguium
How can fungi resist KOH effects
a. Chitin
b. Release of substances
c. Digests KOH as energy
source
d. KOH is a weak reagent
Chitin resists KOH effects
What is true about microccocus spp.?
a. Gram positive cocci in tetrads
b. Gram positive cocci in lancet
c. Gram negative cocci in
tetrads
d. Gram negative cocci in lancet
Gram positive cocci in tetrads
Which of the following exhibits a brown halo in Cystine-Tellurite Blood Agar (CTBA)
a. Corynebacterium diptheriae
b. Bacillus anthracis
c. Listeria monocytogenes
d. Nocardia asteroids
Corynebacterium diptheriae
Mycobacterial disease wherein patient presents “leonine facie” with presence of skin lesions and symmetric nerve damage
a. Tuberculosis
b. Tuberculoid leprosy
c. Lepromatous leprosy
d. Pott’s disease
Lepromatous leprosy
Color reaction to Oxidase positive
a. Indophenol indigo
b. Indophenol purple
c. green d. yellow
Indophenol purple
Which of the products below is detected by Voges-Proskauer test?
a. Acetoin
b. Nitrite
c. Hydrogen sulfide d. Acetic Acid
Acetoin
A TSIA inoculated with an organism gave the following reactions: Acid slant Acid/butt, Gas production, No H2S production. This microorganism is likely?
a. Shigella dysenteriae
b. Pseudomonas aeruginosa
c. Salmonella typhi
d. Klebsiella pneumonia
Klebsiella pneumonia
When grouped according to habitat, which of the following is a true NON-enteric pathogen?
a. Klebsiella pneumoniae
b. Escherichia coli
c. Enterococcus faecalis
d. Yersinia pestis
Yersinia pestis
When grouped according to habitat, which of the following is a true enteric pathogen?
a. Klebsiella pneumoniae
b. Escherichia coli
c. Enterococcus faecalis
d. Yersinia enterocolitica
Yersinia enterocolitica
Which of the following biochemical tests is used to identify non-lactose fermenters?
a. ONPG Test
b. MUG Test
c. Indole Test
d. Phenylalanine deaminase
Test
ONPG Test
Ornithine decarboxylase positive, Indole negative with a characteristic burnt gun powder odor & swarming growth?
a. Proteus mirabilis
b. Escherichia coli
c. Yersinia pestis
d. Salmonella typhi
Proteus mirabilis
Which of the following strains of Escherichia coli is capable of causing Hemolytic Uremic Syndrome (HUS)?
a. EPEC b. ETEC c. EIEC d. EHEC
EHEC
A group of elementary students became ill after eating undercooked ground beef prepared in the school cafeteria. The suspected pathogen, E. coli serotype 0157:H7, is usually recovered using which of the following media?
a. XLD Agar
b. Hektoen-Enteric Agar
c. MacConkey Agar
d. MacConkey Agar with Sorbitol
MacConkey Agar with Sorbitol
I. The function of acid alcohol is to deccolorize acid fast bacilli
II. Preparation - 5ml HCL, 97 mL of 95% absolute ethanol
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE
Both FALSE
I. Nigrosin is used for detection of capsules II. Neufeld is used for capsular antigens
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE
Both TRUE
I. To collect for male urogenital infections, urethral discharge or prostatic fluid may be used.
II. For female urogenital infections, samples can be
collected from the uterine cervix or cervical discharge
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE
Both TRUE
I. Gram positive bacteria retain their primary stain because of their techoic acid and
II. Gram negative bacteria retain their secondary stain because of their large lipopolysaccharide in their cell wall
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE
Both FALSE
I. CAMP test positive result is an arrowhead or bowtie hemolysis.
II. CAMP test stands for Christine, Atkinson, Munch, & Peterson Test
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
First Statement is TRUE, Second Statement is FALSE
I - Coryforms are most likely the possible cause of UTI in alkaline and struvite crystals are present
II - Listeria monocytogens exhibits positive in CAMP
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE
Both Statements FALSe
I - Neisseria gonorrhoeae are catalase and oxidase positive
II - Neisseria elongata is oxidase positive and catalase negative
Both Statements TRUE
I - Neisseria gonorrhoeae and Neisseria meningitis form smooth, uniform grey/brown colonies with a greenish color underneath on primary isolation medium
II - Neisseria gonorrhoeae grows well on thayer martin than in CAP
Both Statements TRUE
I - E. coli H2S & Indole NEGATIVE
II - K. pneumoniae H2S & Urease NEGATIVE
Both Statements TRUE
TSIA reaction of Pseudomonas aeruginosa
a. A/A G H2S (-)
b. K/A G H2S (+)
c. K/A H2S (-)
d. K/K H2S (-)
K/K H2S (-)
Which of the following is the (preferred time) for medical wastes containing body fluids and blood
a. 121 celsius for 15 mins
b. 132 celsius for 15 mins
c. 121 celsius for 4 mins
d. 132 celsius for 4 mins
121 celsius for 15 mins
Which of the following enterics are known to cause diarrhea & are considered pathogens?
a. Salmonella, Shigella, & Yersinia
b. Enterobacter, Klebsiella & Moraxella
c. Acinetobacter, Serratia
d. Enterobacter, Morganella
e.
Proteus, Citrobacter,
Salmonella, Shigella, & Yersinia
Felicity Smoak was admitted to a nearby hospital due to malaise, nonproductive cough & shortness of breath. She had daily fevers, night sweats and a recent 5kg weight loss. She shared that her mother died of tuberculosis when she was only an infant. Liver and bone marrow biopsies shows granulomas with presence of AFB. She is probably infected with?
a. M. tuberculosis
b. M. kansasii
c. M. bovis
d. M. leprae
M. tuberculosis
How is it that fungi can resist the keratin dissolving effects of potassium hydroxide?
a. Fungi contains chitin which resists the effects of KOH
b. Fungi releases substances that counteract the effect of KOH
c. Fungi digests KOH as an energy source thus it is not dissolved
d. KOH is a weak reagent and can only dissolve keratin structures
Fungi contains chitin which resists the effects of KOH
Which of the following compounds do not contribute to the acid-resistance and waxy character of the mycobacterial cell wall
A) N-acetylmuramic acid
B) Cord factor C) WaxD
D) Sulfolipids
N-acetylmuramic acid
What is the next best approach to confirm that an acid-fast bacilli is MTB?
A. Do gram stain technique
B. Recommend the patient for chest
imaging when available
C. Mantoux test should be done and the
physician should be alerted
immediately
D. GeneXpert can be recommended to the patient and the physician in-charge
Mantoux test should be done and the
physician sh
How would you obtain a proper pulmonary specimen for AFS if the patient is 5 years old, ambulatory with low compliance?
A. Instructed the patient to take a deep breath 3 times, hold it momentarily on the 3rd breath, and then cough deeply and vigorously
B.Bronchoscopic aspirations should
be used
C. Aerosol induction is to be done to obtain a proper sputum specimen.
D. Nasal secretions is acceptable since the patient is of a young age
Aerosol induction is to be done to obtain a proper sputum specimen.
An acid-fast smear on the Ziehl-Neelsen stain was reported as 2/50 fields on a 1000x magnification using the WHO/IUATLD standards. How would you report your findings?
A. 1+ B. +2 C. 2+ D.3+
2+
At what temperature does potentially pathogenic fungi need to be in, in order to grow? A. 25C
B. 33C C. 98F D. 128F
98F
Which of the following dye can stain mycobacterium
a. Crystal violet
b. Rose bengal
c. Methylene blue
d. Safranin O
Crystal violet
Which of the following culture media contains antibiotics and selective for pathologic neisseria
a. EMB
b. MacConckey
c. Colystin polymixin? agar
d. Modified Thayer Martin
Modified Thayer Martin
Which of the following stains does this microorganism stain well (picture sng spirochetes)
Silver Nitrate
A 25yr old sexually active man had a history of trading drugs presents to the emergency with a painless 1cm long lesion on the dorsal shaft of his penis linguinal lymphadenopathy is present rpr test result is positive and syphilis is suspected however gram stain reveals no bacteria. T.pallidum is the causative agent of syphilis and cannot be visualized by light microscopy (pota nga pamangkot - sir gian) what do you think is the reason for this?
It has a diameter of < 0.2
Suggest to confirm if acid-fast bacilli is MTB?
A. Gram stain
B. Chest imaging
C. Mantoux test
D. GenXpert
Mantoux test
Brienne Tarth complained of a persistent pruritic rash throughout her body after making a tip to Mexico. She appeared to be well nourished but her physical exam revealed lesions across her nasal bridge area, cheeks and abdomen. If the patient will pay the physician a visit, the physician will likely request for?
a. Biopsy of skin lesions subjected to ziehl-
neelsen
b. Skin lesions swabbed smears stained with crystal violet
c. Blood culture and sensitivity
d. AOTA
Skin lesions swabbed smears stained with crystal violet
Stefan Salvatore was experiencing low grade fever, night sweat, sudden weight loss and was expectorating sputum streaked laden with blood. If the patient will pay the physician a visit, the physician will likely request for?
a. Gram-staining of throat swab smear
b. Kinyoun staining of sputum smear
c. Blood culture and sensitivity
d. AOTA
Kinyoun staining of sputum smear
What is the principle of gram staining?
A. Vita stain
B. Primary stain
C. Differential stain
D. Special stain
Differential stain
Vegetative bacteria in 100 degrees celsius for 15 minutes
a. Pasteurization
b. Boiling
c. Dry Heating d. Incineration
Boiling
Best way to get Specimen
a. Before fibril state spikes
b. Before the administration of
antibiotics
c. On the peak of illness
d. All of the above
All of the above
RACE meaning
Remove, Alarm, Confine and Extinguish/Evacuate
Which describes decontamination
a. Application of a liquid antimicrobial chemical to skin or living tissue
b. Removes,kills, or inactivates all forms of life
c. Removing or neutralizing contaminants that have accumulated on personnel and equipment
d. Eliminates most pathogens but not necessarily all types of microbes
Removing or neutralizing contaminants that have accumulated on personnel and equipment
Optimal temp for growth of most bacterias
30-37
Which of the following bacteria prefer growth on increased CO2?
a. Microaerophiles
b. Mesophiles
c. Capnophilic
d. Facultative Anaerobe
Capnophilic
Which of the following results can be
Obtained from a patient with Acute bacterial pneumonia
a. 1-5 PMN/LPF 20-33 SEC/LPF
gram (+) cocci
b. 80-100
PMN/LPD 0.1
SEC/LPF gram (+) cocci
columnar cells
c.60-95 PMN/LPF 0.1 SEC/LPF
gram + cocci in alveolar macrophages
d.15-15 PMN/LPF 100-130
SEC/LPF gram + cocci in in tetrads
60-95 PMN/LPF 0.1 SEC/LPF
gram + cocci in alveolar macrophages
The epidemiological team of doh has observed that these bacteria have sporadic or epidemic incidence during rainy days as brought by change in weather, complications is usually observed as hypotension and bilateral hemorrhage of adrenal glands commonly known as waterhouse-friedrichsen syndrome.
N. Meningitis
Proper environment for N. gonorrhea
0% O2; 15% CO2
0% 02: 5-10 c02
5-10% 02; 8-10 CO2
15% 02; 5-10% CO2
15% 02; 5-10% CO2
Which of the ff is acceptable preserved specimen
Sputum swab at 4C for 24 hrs
Skin lesion at 22C for 24hrs
Rectal swab at 4c fpr 24hrs
___ 22C for 24hrs
Sputum swab at 4C for 24 hrs
