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MTAP 1 > Bacteria Retake > Flashcards

Bacteria Retake Flashcards

1
Q

Which of the following urine samples is NOT acceptable for bacterial culture?
a. Straight Catheterized Urine
b. Routinely Avoided Urine
c. Midstream Clean Catch Urine
d. Suprapubic Aspiration

A

Routinely avoided urine

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2
Q

What is the proper concentration of the anticoagulant used for blood culture?
a. 0.025% Sodium Polyethanol Sulfonate
b. 0.25% Sodium Polyanathol Sulfonate
c. 0.025% Sodium Polyanaethanol Sulfonate
d. 0.25% Sodium Polyanaethanol Sulfonate

A

0.025% Sodium Polyanaethanol Sulfonate

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3
Q

For at least how many millilitres of blood for culture is collected from a 52 year old immunocompromised patient?
a. 10 mL per set of aerobic & anaerobic bottles
b. 20 mL per set of aerobic & anaerobic bottles-
c. None of the choices are correct
d. Both of the choices are correct

A

20 ml

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4
Q

What swab is used for collection of cervical and vaginal secretions?
a. Alginate b. Cotton c. Rayon d. None

A

Rayon

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5
Q

Specimen collection of CSF
a. Within 1 hour
b. Within 2 hours
c. WIthin 30 mins
d. Within 15 mins

A

Within 15 mins

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6
Q

The following buffered sodium media can contain microorganism at 72 hours:
a. Sodium thiosulfocollate
b. Sodium thioglysulfate
c. Sodium thioglycollate
d. Sodium thiosulfate

A

Sodium Thioglycollate

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7
Q

Which of the following terms are used to describe microorganisms that grow under reduced 0, 10% & increased CO, 10%?
a. Aerobes
b. Anaerobes
c. Microaerophilic d. Capnophiles

A

Microaerophilic

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8
Q

Bsc class used for radioisotopes and 30% of air is exhausted and 70% is recirculated
a. Class I
b. Class II A1
c. Class II A2
d. Class III

A

Class II A2

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9
Q

An MLS intern was tasked to perform gram staining. Upon checking, the intern noticed that the acid alcohol decolorizer is in blue color. What should be done by the intern
a. Report the incident
b. Discard the reagent and
report the incident
c. Repeat staining
d. The intern done it logically
and correct

A

Discard the reagent and report the incident

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10
Q

Components of 100mL Kinyouns Carbolfuchsin
a. 4 g basic fuchsin, 8 g phenol, 20 mL 95% alcohol, 100 mL distilled water
b. 8 g basic fuchsin, 4 g phenol, 100 mL 95% alcohol, 20 mL distilled water
c. 2 g basic fuchsin, 6 g phenol, 20 mL 95% alcohol, 100 mL distilled water
d. 6 g basic fuchsin, 2 g phenol, 100 mL 95% alcohol, 20 mL distilled water

A

4g basic fuchsin, 8g phenol, 20ml 95% alcohol

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11
Q

All of the following bacteria species below are either distinctly acid fast or partially acid fast. Which of the following is not an acid fast bacteria species?
I.Nocardia spp.
II.Rhodococcus spp.
III.Cryptosporidium spp.
IV.Clostridium spp.
a. I only
b. II and III
c. IV only
d. I and IV

A

IV only

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12
Q

Which of the following statements best supports the action of the detergent in Kinyouns method
a. Detergent in the solution enhances the stain’s color
b. It acts as a decontaminant for
other pathogens that may be
present in the specimen
c. Detergent is used to force the
carbolfuchsin into the
mycobacterial cell wall
d. Detergent acts as a mordant
in acid-fast staining

A

Detergent is used to force the carbolfuchsin into the mycobacterial cell wall

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13
Q

How would you describe acid-fast bacteria under the brightfield microscope using your Ziehl-Neelsen stain?
a. -Blue microorganisms on a red background
b. -Red microorganism on a red background
c. -Red microorganisms on a blue background
d. -Blue microorganisms on a blue background

A

Red microorganisms on a blue background

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14
Q

What is the function of the mycolic acid in acid fast bacteria?
a. It renders the bacteria resistant to harsh chemicals
b. It is an exotoxin that helps it multiply once in the host’s body
c. Mycolic acid is what helps the medical technologist determine if it is an acid fast bacteria
d. All statements are true

A

It renders the bacteria resistant to harsh chemicals

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15
Q

Which among the ff is not a common dermatophyte
a. Tinea barbae
b. Tricophyton rubrum
c. Tinea cruris
d. Aspergillus niger

A

Aspergillus niger

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16
Q

Which fungi is found in nails and causes onychomycosis
a. Tinea capitis
b. Tinea corporis
c. Tinea unguium
d. Trichophyton mentagrophytes

A

Tinea unguium

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17
Q

How can fungi resist KOH effects
a. Chitin
b. Release of substances
c. Digests KOH as energy
source
d. KOH is a weak reagent

A

Chitin resists KOH effects

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18
Q

What is true about microccocus spp.?
a. Gram positive cocci in tetrads
b. Gram positive cocci in lancet
c. Gram negative cocci in
tetrads
d. Gram negative cocci in lancet

A

Gram positive cocci in tetrads

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19
Q

Which of the following exhibits a brown halo in Cystine-Tellurite Blood Agar (CTBA)
a. Corynebacterium diptheriae
b. Bacillus anthracis
c. Listeria monocytogenes
d. Nocardia asteroids

A

Corynebacterium diptheriae

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20
Q

Mycobacterial disease wherein patient presents “leonine facie” with presence of skin lesions and symmetric nerve damage
a. Tuberculosis
b. Tuberculoid leprosy
c. Lepromatous leprosy
d. Pott’s disease

A

Lepromatous leprosy

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21
Q

Color reaction to Oxidase positive
a. Indophenol indigo
b. Indophenol purple
c. green d. yellow

A

Indophenol purple

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22
Q

Which of the products below is detected by Voges-Proskauer test?
a. Acetoin
b. Nitrite
c. Hydrogen sulfide d. Acetic Acid

A

Acetoin

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23
Q

A TSIA inoculated with an organism gave the following reactions: Acid slant Acid/butt, Gas production, No H2S production. This microorganism is likely?
a. Shigella dysenteriae
b. Pseudomonas aeruginosa
c. Salmonella typhi
d. Klebsiella pneumonia

A

Klebsiella pneumonia

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24
Q

When grouped according to habitat, which of the following is a true NON-enteric pathogen?
a. Klebsiella pneumoniae
b. Escherichia coli
c. Enterococcus faecalis
d. Yersinia pestis

A

Yersinia pestis

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25
Q

When grouped according to habitat, which of the following is a true enteric pathogen?
a. Klebsiella pneumoniae
b. Escherichia coli
c. Enterococcus faecalis
d. Yersinia enterocolitica

A

Yersinia enterocolitica

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26
Q

Which of the following biochemical tests is used to identify non-lactose fermenters?
a. ONPG Test
b. MUG Test
c. Indole Test
d. Phenylalanine deaminase
Test

A

ONPG Test

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27
Q

Ornithine decarboxylase positive, Indole negative with a characteristic burnt gun powder odor & swarming growth?
a. Proteus mirabilis
b. Escherichia coli
c. Yersinia pestis
d. Salmonella typhi

A

Proteus mirabilis

28
Q

Which of the following strains of Escherichia coli is capable of causing Hemolytic Uremic Syndrome (HUS)?
a. EPEC b. ETEC c. EIEC d. EHEC

A

EHEC

29
Q

A group of elementary students became ill after eating undercooked ground beef prepared in the school cafeteria. The suspected pathogen, E. coli serotype 0157:H7, is usually recovered using which of the following media?
a. XLD Agar
b. Hektoen-Enteric Agar
c. MacConkey Agar
d. MacConkey Agar with Sorbitol

A

MacConkey Agar with Sorbitol

30
Q

I. The function of acid alcohol is to deccolorize acid fast bacilli
II. Preparation - 5ml HCL, 97 mL of 95% absolute ethanol
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE

A

Both FALSE

31
Q

I. Nigrosin is used for detection of capsules II. Neufeld is used for capsular antigens
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE

A

Both TRUE

32
Q

I. To collect for male urogenital infections, urethral discharge or prostatic fluid may be used.
II. For female urogenital infections, samples can be
collected from the uterine cervix or cervical discharge
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE

A

Both TRUE

33
Q

I. Gram positive bacteria retain their primary stain because of their techoic acid and
II. Gram negative bacteria retain their secondary stain because of their large lipopolysaccharide in their cell wall
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE

A

Both FALSE

34
Q

I. CAMP test positive result is an arrowhead or bowtie hemolysis.
II. CAMP test stands for Christine, Atkinson, Munch, & Peterson Test
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE

A

First Statement is TRUE, Second Statement is FALSE

35
Q

I - Coryforms are most likely the possible cause of UTI in alkaline and struvite crystals are present
II - Listeria monocytogens exhibits positive in CAMP
a. First Statement is TRUE, Second Statement is FALSE
b. First Statement is FALSE, Second Statement is TRUE
c. Both Statements TRUE
d. Both Statements FALSE

A

Both Statements FALSe

36
Q

I - Neisseria gonorrhoeae are catalase and oxidase positive
II - Neisseria elongata is oxidase positive and catalase negative

A

Both Statements TRUE

37
Q

I - Neisseria gonorrhoeae and Neisseria meningitis form smooth, uniform grey/brown colonies with a greenish color underneath on primary isolation medium
II - Neisseria gonorrhoeae grows well on thayer martin than in CAP

A

Both Statements TRUE

38
Q

I - E. coli H2S & Indole NEGATIVE
II - K. pneumoniae H2S & Urease NEGATIVE

A

Both Statements TRUE

39
Q

TSIA reaction of Pseudomonas aeruginosa
a. A/A G H2S (-)
b. K/A G H2S (+)
c. K/A H2S (-)
d. K/K H2S (-)

A

K/K H2S (-)

40
Q

Which of the following is the (preferred time) for medical wastes containing body fluids and blood
a. 121 celsius for 15 mins
b. 132 celsius for 15 mins
c. 121 celsius for 4 mins
d. 132 celsius for 4 mins

A

121 celsius for 15 mins

41
Q

Which of the following enterics are known to cause diarrhea & are considered pathogens?
a. Salmonella, Shigella, & Yersinia
b. Enterobacter, Klebsiella & Moraxella
c. Acinetobacter, Serratia
d. Enterobacter, Morganella
e.
Proteus, Citrobacter,

A

Salmonella, Shigella, & Yersinia

42
Q

Felicity Smoak was admitted to a nearby hospital due to malaise, nonproductive cough & shortness of breath. She had daily fevers, night sweats and a recent 5kg weight loss. She shared that her mother died of tuberculosis when she was only an infant. Liver and bone marrow biopsies shows granulomas with presence of AFB. She is probably infected with?
a. M. tuberculosis
b. M. kansasii
c. M. bovis
d. M. leprae

A

M. tuberculosis

43
Q

How is it that fungi can resist the keratin dissolving effects of potassium hydroxide?
a. Fungi contains chitin which resists the effects of KOH
b. Fungi releases substances that counteract the effect of KOH
c. Fungi digests KOH as an energy source thus it is not dissolved
d. KOH is a weak reagent and can only dissolve keratin structures

A

Fungi contains chitin which resists the effects of KOH

44
Q

Which of the following compounds do not contribute to the acid-resistance and waxy character of the mycobacterial cell wall
A) N-acetylmuramic acid
B) Cord factor C) WaxD
D) Sulfolipids

A

N-acetylmuramic acid

45
Q

What is the next best approach to confirm that an acid-fast bacilli is MTB?
A. Do gram stain technique
B. Recommend the patient for chest
imaging when available
C. Mantoux test should be done and the
physician should be alerted
immediately
D. GeneXpert can be recommended to the patient and the physician in-charge

A

Mantoux test should be done and the
physician sh

46
Q

How would you obtain a proper pulmonary specimen for AFS if the patient is 5 years old, ambulatory with low compliance?
A. Instructed the patient to take a deep breath 3 times, hold it momentarily on the 3rd breath, and then cough deeply and vigorously
B.Bronchoscopic aspirations should
be used
C. Aerosol induction is to be done to obtain a proper sputum specimen.
D. Nasal secretions is acceptable since the patient is of a young age

A

Aerosol induction is to be done to obtain a proper sputum specimen.

47
Q

An acid-fast smear on the Ziehl-Neelsen stain was reported as 2/50 fields on a 1000x magnification using the WHO/IUATLD standards. How would you report your findings?
A. 1+ B. +2 C. 2+ D.3+

A

2+

48
Q

At what temperature does potentially pathogenic fungi need to be in, in order to grow? A. 25C
B. 33C C. 98F D. 128F

A

98F

49
Q

Which of the following dye can stain mycobacterium
a. Crystal violet
b. Rose bengal
c. Methylene blue
d. Safranin O

A

Crystal violet

50
Q

Which of the following culture media contains antibiotics and selective for pathologic neisseria
a. EMB
b. MacConckey
c. Colystin polymixin? agar
d. Modified Thayer Martin

A

Modified Thayer Martin

51
Q

Which of the following stains does this microorganism stain well (picture sng spirochetes)

A

Silver Nitrate

52
Q

A 25yr old sexually active man had a history of trading drugs presents to the emergency with a painless 1cm long lesion on the dorsal shaft of his penis linguinal lymphadenopathy is present rpr test result is positive and syphilis is suspected however gram stain reveals no bacteria. T.pallidum is the causative agent of syphilis and cannot be visualized by light microscopy (pota nga pamangkot - sir gian) what do you think is the reason for this?

A

It has a diameter of < 0.2

53
Q

Suggest to confirm if acid-fast bacilli is MTB?
A. Gram stain
B. Chest imaging
C. Mantoux test
D. GenXpert

A

Mantoux test

54
Q

Brienne Tarth complained of a persistent pruritic rash throughout her body after making a tip to Mexico. She appeared to be well nourished but her physical exam revealed lesions across her nasal bridge area, cheeks and abdomen. If the patient will pay the physician a visit, the physician will likely request for?
a. Biopsy of skin lesions subjected to ziehl-
neelsen
b. Skin lesions swabbed smears stained with crystal violet
c. Blood culture and sensitivity
d. AOTA

A

Skin lesions swabbed smears stained with crystal violet

55
Q

Stefan Salvatore was experiencing low grade fever, night sweat, sudden weight loss and was expectorating sputum streaked laden with blood. If the patient will pay the physician a visit, the physician will likely request for?
a. Gram-staining of throat swab smear
b. Kinyoun staining of sputum smear
c. Blood culture and sensitivity
d. AOTA

A

Kinyoun staining of sputum smear

56
Q

What is the principle of gram staining?
A. Vita stain
B. Primary stain
C. Differential stain
D. Special stain

A

Differential stain

57
Q

Vegetative bacteria in 100 degrees celsius for 15 minutes
a. Pasteurization
b. Boiling
c. Dry Heating d. Incineration

A

Boiling

58
Q

Best way to get Specimen
a. Before fibril state spikes
b. Before the administration of
antibiotics
c. On the peak of illness
d. All of the above

A

All of the above

59
Q

RACE meaning

A

Remove, Alarm, Confine and Extinguish/Evacuate

60
Q

Which describes decontamination
a. Application of a liquid antimicrobial chemical to skin or living tissue
b. Removes,kills, or inactivates all forms of life
c. Removing or neutralizing contaminants that have accumulated on personnel and equipment
d. Eliminates most pathogens but not necessarily all types of microbes

A

Removing or neutralizing contaminants that have accumulated on personnel and equipment

61
Q

Optimal temp for growth of most bacterias

A

30-37

62
Q

Which of the following bacteria prefer growth on increased CO2?
a. Microaerophiles
b. Mesophiles
c. Capnophilic
d. Facultative Anaerobe

A

Capnophilic

63
Q

Which of the following results can be
Obtained from a patient with Acute bacterial pneumonia
a. 1-5 PMN/LPF 20-33 SEC/LPF
gram (+) cocci
b. 80-100
PMN/LPD 0.1
SEC/LPF gram (+) cocci
columnar cells
c.60-95 PMN/LPF 0.1 SEC/LPF
gram + cocci in alveolar macrophages
d.15-15 PMN/LPF 100-130
SEC/LPF gram + cocci in in tetrads

A

60-95 PMN/LPF 0.1 SEC/LPF
gram + cocci in alveolar macrophages

64
Q

The epidemiological team of doh has observed that these bacteria have sporadic or epidemic incidence during rainy days as brought by change in weather, complications is usually observed as hypotension and bilateral hemorrhage of adrenal glands commonly known as waterhouse-friedrichsen syndrome.

A

N. Meningitis

65
Q

Proper environment for N. gonorrhea
0% O2; 15% CO2
0% 02: 5-10 c02
5-10% 02; 8-10 CO2
15% 02; 5-10% CO2

A

15% 02; 5-10% CO2

66
Q

Which of the ff is acceptable preserved specimen
Sputum swab at 4C for 24 hrs
Skin lesion at 22C for 24hrs
Rectal swab at 4c fpr 24hrs
___ 22C for 24hrs

A

Sputum swab at 4C for 24 hrs

MTAP 1 (13 decks)

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