Hema Flashcards
Collection and Handling of Blood Specimens
What is the possible complication if you pushed too deep the lancet when doing skin puncture?
a. Osteoporosis
b. Osteoarthritis
c. Osteosarcoma
d. Osteomyelitis
Osteomyelitis
In skin puncture, why is it important to warm the puncture site before collecting the sample?
To prevent contamination of lymphatic fluid from the blood
b. To avoid pain when punctured
c. To ensure the free flow of blood
d. To have a good picture of cells during
microscopie examination
To ensure the free flow of blood
When counting in hemocytometer, what will be the difference of the total cells counted in each side?
a. <5%
b. >5%
c. >10%
d. <10%
-<10% (Rodaks)
Which of the following RBC diluting fluid requires the use of spectrophotometer?
a. Hayem’s fluid b. Bethel’s fluid c. Toisson’s fluid d. Gower’s fluid
-Gower’s fluid
What counting chamber is used for basophil and eosinophil count?
a. Fuchs-Rosenthal
b. Levy-Hausser
c. Thoma-Zeiss
d. Addis
Fuchs-Rosenthal
What is the reason why we need to correct the WBC count when nucleated RBCs are seen in PBS?
a. Nucleated RBs are confused with giant thrombocytes
b. Nucleated RBCs are counted as leukocytes
c. The WBC count would be falsely lower
d. The RBC count is too low
Nucleated RBCs are counted as leukocytes
A 1:200 dilution of patient’s sample was made and 330 red cells were counted in an area of 0.2 mm. What is the RBC count?
a. 1.68 x 10’/L
b. 3.30 x 10”/L
c. 6.60 × 10’L
d. 3.36 x 104L
-3.30 x 10”/L (330x200/0.2x0.1)
Which of the following is true about the Coulter principle?
a. Isotonic solutions cannot conduct electricity
b. Isotonic solutions conduct electricity better
than cells do
c. Conductivity varies proportionally to the
number of cells
d. Cells conduct electricity better than saline
does
-Isotonic solutions conduct electricity better than cells do
What hematology analyzer uses multi-angle polarized scatter separation (MAPPS) to perform leukocyte count?
a. Sysmex E-500
b. Hemalog D
c. Coulter STKS
d. Cell-DYN 3000
Cell-DYN 3000
Which of the Coulter hematology system produces complete differential with interpretative result?
a. Coulter Counter model S-Plus series
b. Coulter MAXM
c. Coulter ONYX
d. Coulter STKR
-Coulter STKR
What light scatter angle can measure the cells based on reflection and refraction of internal components?
a. Forward light scatter
b. Forward low-angle light scatter
c. Forward high-angle scatter
d. Orthogonal light scatter
Orthogonal light scatter
In hematocrit determination, what will be the result if there is insufficient centrifigation?
a. Falsely decrease hematocrit value
b. Falselv increase hematocrit value
c. Increase hematocrit result by 30%
d. No effect on hematocrit value
Falselv increase hematocrit value
Which of the following conditions is associated with decreased OFT?
a. Hereditary spherocytosis
b. Acquired hemolytic anemia
c. Hemolytic disease of the newborn
d. Sickle cell anemia
Sickle cell anemia
What RBC index is not affected when the hematocrit is falsely elevated?
a. MCH
b. MCV
c. MCHC
d. RDW
MCH
Which of the following factors has no effect in erythrocyte sedimentation rate?
a. Anisocytosis, poikilocytosis
b. Caliber of the tube
c. Blood drawn in sodium citrate
d. Plasma proteins
Blood drawn in sodium citrate
Which condition will shift the oxyhemoglobin dissociation curve to the right?
a. Alkalosis
b. Acidosis
c. Multiple blood transfusions
d. Increased quantities of hemoglobin C
Acidosis