Hema Retake Flashcards
Total blood volume in an adult
5-6 liters
Micro sample collection only applies to infants, young children, adult with poor veins
should not be used on adult
Used in ESR
3.8% sodium citrate
Produces a bluish background on blood smear when stained with Wright’s or Leishman’s stain
Heparin
Most reliable criteria for estimation of cell lineage
Pattern of nuclear chromatin
Ability of the lens to delineate detail in a specimen
Resolution
Counting chamber for standardization of vaccine
Petroff-Hausser
Counting chamber for eosinophil and basophil
Fuchs-Rosenthal
Diluting fluid used for spectrophotometry
Gower’s Fluid
Used in cases of emergency when no other diluting fluids are available
NSS
Bone marrow cellularity
Aspiration
Multi-lineage cytokine
IL-1
earliest recognizable erythrocytic lineage
Rubriblast
start of Hb formation
Prorubricyte
last stage capable of cell division
Rubricyte
last stage with nucleus
Metarubricyte
stage that is normally seen in the peripheral blood
Reticulocyte
index of effective erythropoiesis
Reticulocyte count
RPI more than or equal to 3
evidence of hemolytic anemia
Generation of ATP
Embden-Meyerhof Pathway
Production of 2,3 DPG that affects the affinity of hemoglobin to oxygen
Leubering-Rapoport Pathway
Variation of Hb content
Anisochromasia
Seen in alcoholic cirrhosis, abetalipoproteinemia, post-splenectomy, PK deficiency, & LCAT deficiency
Spurr cell (Acanthocyte)
PK deficiency
Echinocyte
G6PD
Degmacyte (bite cell)
IDA
Elliptocyte (cigar)
Sickle cell anemia
Drepanocyte
Scattered inclusion that appears homogeneously
Basophilic stipplings
DNA mitotic spindle remnant of microtubules
Cabot ring
Flat, blue-gray pieces, often in groups of four
Papenheimer bodies
Small, dense, round inclusion, single or multiple
Howell-Jolly Bodies
Predominant Hb in fetal life
Hb F
zero degree
cell size
10 degree
Cell structure
90 degree
lobularity
bacterial infection
Neutrophil
allergy
Basophil
macrophage precursor
Monocyte
parasite infection
Eosinophil
antibody
Lymphocyte
not included in the extramedullary hematopoiesis (meaning its the formation of blood cells outside the bone marrow)
Bone marrow
decreased serum iron and increased TIBC
Iron Deficiency Anemia
valine replaces glutamic acid at the 6th amino acid on beta chain
Hemoglobin S
increased ESR
Titling of tube
Enzyme that is most likely to elicit hemolytic anemia
PK (pyruvate kinase)
Sucrose lysis positive
PNH (paroxysmal nocturnal hemoglobinuria)
Not directly determined in an automated analyzer
Hematocrit
Dense azurophilic granulation in all WBCs except neutrophils, associated with gargoylism
Alder Reilly Anomaly
Irregular size inclusion bodies similar to Dohle-amato bodies, autosomal disorder with presence of inclusion in PMN and monocyte with a giant PLT and low PLT count or thrombocytopenia
May-Hegglin Anomaly
Failure of the nucleus of the neutrophils to lobulate or segment something pince-nez
Pelger-Huet
Recurrent chronic inability of neutrophil to kill ingested bacteria that cannot make hydrogen peroxide
Chronic Granulomatous Disease
Characterized by the presence of peroxidase positive granules and inclusion which appear to be abnormal lysosomes
Chediak Higashi
Least seen in peripheral blood
Basophil
Best estimation of wbc count in blood smear
Absolute count
Best source: active morrow mag aspirate 20 yr old patient
Iliac crest
Anemia of renal failure causes
Decrease production of erythropoiesis
MCV= 76 MCH= 26 MCHC= 27
Microcytic, Normochromic
Absence or deficiency of one globin chain
Thalassemia
Patients undergoing cancer chemotherapy
Myelophthisic anemia
Deficiency causes niemann’s pick disease
Sphingomyelinase
Why is there a need to correct wbc for NRBC?
NRBC can be miscount as WBC due to appearance
Excess iron in an erythroblast
Sideroblast
What can cause anemia associated with neurological symptoms?
Vitamin B12 deficiency
No neurologic manifestations
Folic Acid Deficiency
Failure of erythroid something
Pure red cell aplasia