HTMLE Progress Flashcards
First clinical laboratory in the Philippines:
A. San Lazaro Hospital
B. Research Institute for Tropical Medicine
C. Manila Public Health Laboratory
D. National Reference Laboratory
C. Manila Public Health Laboratory
Introduced medical technology practice in the Philippines after World War II:
A. Dr. Pio de Roda
B. Dr. Prudencia Sta. Ana
C. Dr. Mariano Icasiano
D. 26th Medical Laboratory of the 6th US Army
D. 26th Medical Laboratory of the 6th US Army
RA 5527 consists of:
A. 30 sections
B. 32 sections
C. 50 sections
D. 52 sections
B. 32 sections
Which of the following amends RA 5527 on June 11, 1978?
A. RA 6138
B. PD 498
C. PD 1534
C. PD 1534
All are sections of RA 5527 amended by PD 1534, except:
A. Section 3
B. Section 8
C. Section 13
D. Section 18
D. Section 18
All are qualifications of the Board of Medical Technology, except:
A. Filipino citizen
B. Good moral character
C. Qualified Pathologist, or a duly registered MT
D. In practice of laboratory medicine or MT for at least 5 years prior to his appointment
E. Not a member of the faculty of any MT school for at least 2 years prior to appointment
E. Not a member of the faculty of any MT school for at least 2 years prior to appointment
In practice of laboratory medicine or MT for at least 10 years prior to his appointment.
Board of Medical Technology term of office:
A. Hold office for ONE (1) YEAR after appointments or until their successors shall have been appointed and duly qualified
B. Hold office for THREE (3) YEARS after appointments or until their successors shall have been appointed and duly qualified
C. Hold office for FIVE (5) YEARS after appointments or until their successors shall have been appointed and duly qualified
D. Hold office for SEVEN (7) YEARS after appointments or until their successors shall have been appointed and duly qualified
B. Hold office for THREE (3) YEARS after appointments or until their successors shall have been appointed and duly qualified
Minor subject (10%) in the MT Board Exam:
A. Clinical Chemistry
B. Hematology
C. Immunology, Serology & Blood Banking
D. Clinical Microscopy
D. Clinical Microscopy
In order to pass the MT examination, a candidate must obtain a general average of at least ___ in the written test.
A. General average of at least 50%
B. General average of at least 65%
C. General average of at least 70%
D. General average of at least 75%
D. General average of at least 75%
Refresher course for applicants who have failed the Board Examination for the ___ time.
A. First time
B. Second time
C. Third time
D. Fourth time
C. Third time
Certificate of Registration as Medical Technologist is issued to any successful applicant who has attained the age of:
A. 18
B. 19
C. 20
D. 21
D. 21
Revocation:
A. Unanimous vote (3/3)
B. Majority vote (2/3)
A. Unanimous vote (3/3)
Suspension:
A. Unanimous vote (3/3)
B. Majority vote (2/3)
B. Majority vote (2/3)
Which of the following can only be done in a tertiary category laboratory?
A. Crossmatching
B. Routine chemistry
C. Routine hematology
D. Special hematology
D. Special hematology
Start of renewal of Clinical Laboratory License:
A. October 1
B. November 1
C. December 1
D. January 1
A. October 1
Non–hospital based clinical laboratories shall file applications for renewal of LTO beginning on the FIRST DAY OF OCTOBER UNTIL THE LAST DAY OF NOVEMBER of the current year.
The LICENSE TO OPERATE (LTO) issued to the CLINICAL LABORATORY is valid for:
A. One year
B. Two years
C. Three years
D. Five years
A. One year
Acceptable patient identifiers include:
A. Patient’s name, gender, medical record number
B. Patient’s name, gender, date of birth
C. Patient’s name, requesting physician, medical record number
D. Patient’s name, date of birth, medical record number
D. Patient’s name, date of birth, medical record number
LICENSE TO OPERATE (LTO) Blood Service Facilities is valid for a period of ____ years.
A. 1 year
B. 2 years
C. 3 years
D. 5 years
C. 3 years
According to DOH A.O. 2020-0031, certificate of accreditation of laboratories for DRINKING WATER ANALYSIS is valid for __ year(s) and expires on the last day of December. TAKE NOTE OF YEAR 2020 DOH REVISION.
A. One year
B. Two years
C. Three years
D. Five years
C. Three years
Part of drinking water analysis, except:
A. Calcium
B. Fecal coliform test
C. Sodium
D. Potassium
D. Potassium
License and accredit drug testing centers in each province and city:
A. DOH
B. PDEA
C. PNP
D. PRC
A. DOH
Methylenedioxymethamphetamine (MDMA):
A. Shabu
B. Meth
C. Ice
D. Ecstasy
D. Ecstasy
Random drug test:
A. Applicants for firearm’s license
B. Officers and members of the military, police and other law enforcers
C. Students of secondary and tertiary schools
D. Candidates for public office whether appointed or elected both in the national or local government
C. Students of secondary and tertiary schools
A drug test is valid for:
A. Six weeks
B. Six months
C. One year
D. Two years
C. One year
All results of HIV/AIDS testing shall be confidential and shall be released only to the following persons, except:
A. Person who submitted himself/herself to such test
B. Boyfriend of the patient
C. Either parent of a minor child who has been tested
D. Legal guardian in the case of insane persons or orphans
Person authorized to receive such results in conjunction with the AIDSWATCH program
B. Boyfriend of the patient
The Professional Regulation Commission, otherwise known as the PRC, is a ____-man commission attached to office of the President for general direction and coordination. [Currently, to DOLE]
A. Two-man
B. Three-man
C. Four-man
D. Five-man
B. Three-man
The current (September 2022) PRC CHAIRPERSON is:
A. Marilyn Barza
B. Teofilo Pilando
C. Charito Zamora
D. Jose Cueto
C. Charito Zamora
If a medical technologist was not able to complete the number of CPD units upon renewal of PRC license, he may sign a/an: [ONLY UP TO DECEMBER 2023]
A. Affidavit of undertaking
B. Certificate of exemption
C. Certificate of registration
D. Suspension order
A. Affidavit of undertaking
An act done to avoid harming the patients:
A. Beneficence
B. Non-maleficence
C. Autonomy
D. Justice
B. Non-maleficence
Obligation of MT to the patient:
A. Compliance to PRC
B. Compliance to the department of health
C. Comprehensive health education
D. Strive for excellence in professional practice
D. Strive for excellence in professional practice
All traits are mentioned in the MT CODE OF ETHICS, except:
A. Honesty
B. Humility
C. Integrity
D. Reliability
B. Humility
Fill in the blank (Code of Ethics): Be dedicated to ____.
A. Fairness to all and in a spirit of brotherhood toward other members of the profession
B. Law and shall not participate in illegal work
C. Responsibilities inherent to being a professional
D. Use of clinical laboratory science to promote life and benefit mankind
D. Use of clinical laboratory science to promote life and benefit mankind
Continuing Professional Development Act of 2016:
A. RA 7719
B. RA 9288
C. RA 10912
D. RA 7170
C. RA 10912
Published standards for GOOD LABORATORY PRACTICE:
A. CDC
B. DOH
C. PRC
D. WHO
D. WHO
Components of quality assurance:
A. Pre-analytical variables
B. Analytical variables
C. Post-analytical variables
D. All of these
D. All of these
An example of cellular adaptation is:
A. Edema
B. Inflammation
C. Neoplasia
D. Dysplasia
D. Dysplasia
Low temperature:
A. Enhance fixation
B. Retard fixation
C. Variable
D. No effect
B. Retard fixation
Stopping all cellular activities so that the cells can be viewed under the microscope as if they are still in their original living state:
A. Decalcification
B. Embedding
C. Fixation
D. Staining
C. Fixation
Fixative for electron microscopy:
A. Carnoy’s
B. Formalin
C. Glutaraldehyde
D. Zenker
C. Glutaraldehyde
“GOP KZ”
Glutaraldehyde, Osmium tetroxide, Paraformaldehyde, Karnovsky’s, Zamboni
Most common and fastest decalcifying agent used:
A. Formic acid
B. Hydrochloric acid
C. Nitric acid
D. Sulfurous acid
C. Nitric acid
For most instances, dehydration starts by placing the fixed specimen in:
A. 70% ethyl alcohol
B. 95% ethyl alcohol
C. Absolute alcohol
D. Xylene
A. 70% ethyl alcohol
Transition step between dehydration and infiltration with the embedding medium:
A. Fixation
B. Clearing
C. Infiltration
D. Mounting
B. Clearing
Enclosed tissue processor:
A. Mechanical transfer
B. Dip and dunk
C. Fluid transfer
D. Tissue transfer
C. Fluid transfer
What are the processes (IN ORDER) done by the automatic tissue processor:
A. Fixation, clearing, dehydration and infiltration
B. Fixation, infiltration, dehydration and clearing
C. Fixation, dehydration, clearing and infiltration
D. Fixation, dehydration, infiltration and clearing
C. Fixation, dehydration, clearing and infiltration
These knives are used to cut block for ELECTRON MICROSCOPY (EM):
A. Disposal blades
B. Steel knives
C. Diamond or glass knives
D. Magnetic blades
C. Diamond or glass knives
Sections fail to form ribbons:
A. Hard spot in tissue due to calcium
B. Sections are too thick
C. Paraffin is impure
D. Knife edge is dirty
B. Sections are too thick
Effect of basic pH to ripening process:
A. Slower oxidizing process
B. More rapid oxidizing process
C. Variable
D. No effect
B. More rapid oxidizing process
RETICULIN FIBERS IN GOMORI’S silver impregnation stain:
A. Black
B. Blue
C. Red
D. Green
A. Black
A stain containing silver nitrate for demonstration of spirochetes:
A. Fite-Faraco
B. Warthin-Starry
C. Masson-Fontana
D. Mallory’s PTAH
B. Warthin-Starry
Frozen sections are stained by hand because:
A. Staining is more accurate
B. Prevent overstaining
C. Faster for one or a few individual sections
D. Predictable colors
C. Faster for one or a few individual sections
_______ are raised against specific cellular _____ and then conjugated with a _______.
A. Antigen, antibody, visual marker
B. Visual marker, antigen, antibody
C. Antibody, antigen, visual marker
D. Visual marker, antibody, antigen
C. Antibody, antigen, visual marker
Gastrointestinal specimens, except:
A. Gastric lavage
B. Gastric brush
C. Fine needle aspirate (submucosal lesions)
D. Induced vomiting
D. Induced vomiting
Liquid-based cytology samples:
A. Body fluids
B. Touch imprint
C. Brush sampling
D. Skin scrape
A. Body fluids
It is considered to be a most sensitive and specific reagent for lipid staining:
A. Sudan III
B. Sudan IV
C. Sudan Black
D. Oil Red O
C. Sudan Black
One measure of the efficiency of a surgical pathology service:
A. Rapidity of accurate reporting the diagnosis to clinicians
B. Rapidity of tissue processing
C. Sufficiency of tissue preservation
D. Sufficiency of reagents and standards
A. Rapidity of accurate reporting the diagnosis to clinicians
Objective of a quality assurance program in histopathology:
A. Ensure to process the tissues
B. Ensure acceptable service
C. Ensure to follow standards
D. Ensure the completeness, accuracy and timeliness of a histopathology report
D. Ensure the completeness, accuracy and timeliness of a histopathology report
Primary objective in quality and safety control programs in histopathology laboratories:
A. Correctness of interpretation of reports
B. Ensure correct sampling
C. Ensure accurate treatment to patient
D. Promotion of health and safety of patient, laboratory personnel and environment
D. Promotion of health and safety of patient, laboratory personnel and environment
Which of the following fixatives contains picric acid, formalin, and acetic acid?
A. Zenker
B. Helly
C. Bouin
D. Zamboni
C. Bouin
The volume of fixative should exceed the volume of the tissue by:
A. 1 to 2 times
B. 5 to 10 times
C. 10 to 20 times
D. 25 to 50 times
C. 10 to 20 times
Which of the following fixatives contains formalin, potassium dichromate, and mercuric chloride?
A. Zenker
B. Helly
C. Carnoy
D. Orth
B. Helly
Precipitate left in tissues that have been fixed in solutions containing mercuric chloride may be removed by immersion in:
A. Running water
B. Sodium thiosulfate
C. Weak ammonia water
D. Iodine
D. Iodine
Commercial stock formaldehyde solutions contain:
A. 4% formaldehyde
B. 10% formaldehyde
C. 37 to 40% formaldehyde
D. 98 to 100% formaldehyde
C. 37 to 40% formaldehyde
Important:
Commercial stock solution: 37 to 40% formaldehyde
As fixing fluid: 10% formalin
Formalin pigment may be removed from tissue by:
A. Running water
B. Alcoholic iodine
C. Alcoholic picric acid
D. Potassium permanganate
C. Alcoholic picric acid
To prepare a 10% solution of formalin, which of the following amounts of water should be added to 100 mL of stock formaldehyde:
A. 1,000 mL
B. 900 mL
C. 450 mL
D. 10 mL
B. 900 mL
Important: 1:10 dilution
1 part 40% formaldehyde
9 parts water
TV = 10
Carnoy fluid is prepared with acetic acid, alcohol, and:
A. Chloroform
B. Formalin
C. Acetone
D. Osmium tetroxide
A. Chloroform
The first and most important procedure in the preparation of a tissue for microscopic examination is the choice of:
A. Fixative
B. Dehydrating agent
C. Clearing agent
D. Staining technique
A. Fixative
Generally, an increase in the temperature of the fixative solution:
A. Decreases the tissue autolysis
B. Decreases the fixative penetration
C. Increases the speed of fixation
D. Increases the volume of fixative needed
C. Increases the speed of fixation
Which of the following may cause tissue to become overhardened?
A. Prolonged fixation
B. Abbreviated fixation
C. Inadequate dehydration
D. Incomplete clearing
A. Prolonged fixation
To prevent polymerization of formaldehyde, which of the following is added to the commercial stock solutions?
A. Methyl alcohol
B. Formic acid
C. Paraformaldehyde
D. Sodium phosphate
A. Methyl alcohol
Which of the following is a dehydrating agent?
A. Formalin
B. Xylene
C. Benzene
D. Alcohol
D. Alcohol
A clearing agent for use in processing tissues for paraffin embedding must be miscible with the:
A. Fixative and paraffin
B. Dehydrant and paraffin
C. Fixative and dehydrant
D. Paraffin and water
B. Dehydrant and paraffin
Dioxane is a reagent that can be used:
A. For both fixing and dehydrating tissues
B. For both dehydrating and clearing tissues
C. In very small volume ratios
D. For long periods without changing
B. For both dehydrating and clearing tissues
A reagent that CANNOT be used for dehydrating tissue is:
A. Benzene
B. Absolute alcohol
C. Dioxane
D. Acetone
A. Benzene
Which of the following chemicals is NOT a clearing agent?
A. Chloroform
B. Dioxane
C. Ethanol
D. Xylene
C. Ethanol
The process of removing water from tissue is called:
A. Dehydration
B. Reduction
C. Oxidation
D. Clearing
A. Dehydration
The dehydration and clearing steps can be omitted when using:
A. Celloidin
B. Epoxy resin
C. Glycol methacrylate
D. Water-soluble wax
D. Water-soluble wax
During microtomy, it is noted that most of the tissue is very hard and shrunken. One of the first things to check to prevent its happening in the future is the:
A. Presence of water in the clearing agent
B. pH of the fixative
C. Temperature of the infiltrating paraffin
D. Freshness of the reagents on the processor
C. Temperature of the infiltrating paraffin
The temperature of the oven used to maintain a supply of melted paraffin for embedding tissue should be about:
A. 43C
B. 43F
C. 60C
D. 60F
C. 60C
When using a microscope with a x10 ocular and a x40 objective, the total magnification is approximately:
A. 100
B. 400
C. 1,000
D. 4,000
B. 400
Important:
Total magnification (TM)
= magnification of objective x magnification of ocular
= 10 x 40
= 400
The microwave oven creates heat in staining solutions by:
A. Convection
B. Conduction
C. Nonionizing radiation
D. Electrolytic action
C. Nonionizing radiation
Harris hematoxylin is used on tissue sections to stain:
A. Fat
B. Glycogen
C. Nuclei
D. Cytoplasm
C. Nuclei
Ripening of hematoxylin is a process of:
A. Hydrolysis
B. Oxidation
C. Mordanting
D. Reduction
B. Oxidation
The active staining ingredient in ripened hematoxylin solutions is:
A. Hematin
B. Hematein
C. Hematoxylin
D. Hemosiderin
B. Hematein
The most important step in regressive hematoxylin staining is:
A. Postmordanting in picric acid
B. Use of hematoxylin containing acetic acid
C. Differentiation in acid-alcohol
D. Washing in water after the hematoxylin
C. Differentiation in acid-alcohol
Mordants are used to:
A. Change the refractive index of the tissue
B. Help differentiate stains
C. Link tissue constituents more closely to the dye
D. Oxidize staining solutions
C. Link tissue constituents more closely to the dye
The combination of a dye and a mordant is called a/an:
A. Base
B. Accelerator
C. Lake
D. Buffer
C. Lake
Mercuric oxide (or sodium iodate) is used in Harris hematoxylin to:
A. Form hematein
B. Prevent oxidation
C. Serve as the mordant
D. Stabilize the solution
A. Form hematein
DNA can be demonstrated with:
A. Eosin
B. Pyronin
C. Feulgen reaction
D. Fast green
C. Feulgen reaction
The mordant in Weigert hematoxylin is:
A. Iron
B. Aluminum
C. Mercury
D. Tungsten
A. Iron
Acetic acid is added to Harris hematoxylin to:
A. Keep heterochromatin from staining
B. Make nuclear staining more specific
C. Ripen the hematoxylin
D. Form a dye lake
B. Make nuclear staining more specific
Which of the following is stained rose by the methyl green-pyronin (MGP) technique?
A. Heterochromatin
B. DNA
C. RNA
D. Golgi apparatus
C. RNA
Differentiating in the H&E stain is an example of using:
A. Excess mordant
B. Weak acid
C. Oxidizers
D. Buffers
B. Weak acid
The Feulgen reaction demonstrates:
A. DNA only
B. RNA only
C. Both DNA and RNA
D. Phosphoric acid groups
A. DNA only
Sections for special stains have been accidentally stained with hematoxylin. To remove the hematoxylin, place the sections in:
A. Acid alcohol
B. Dilute ammonia
C. Lithium carbonate
D. Isopropyl alcohol
A. Acid alcohol
Glycogen is best demonstrated by the use of:
A. Crystal violet
B. Mayer mucicarmine
C. PAS with and without diastase
D. Alcian blue with and without hyaluronidase
C. PAS with and without diastase
Amyloid can be demonstrated with:
A. Congo red
B. Mayer mucicarmine
C. Cresyl echt violet
D. Alcian blue
A. Congo red
Which of the following methods best demonstrates elastic tissue?
A. Verhoeff
B. Silver impreganation
C. Gomori trichrome
D. PAS
A. Verhoeff
The oil red O stain requires which of the following sections?
A. Paraffin
B. Celloidin
C. Frozen
D. Plastic
C. Frozen
The oil red O stain might be used to demonstrate:
A. Rhabdomyosarcomas
B. Leiomyosarcomas
C. Liposarcomas
D. Adenocarcinomas
C. Liposarcomas
The best stain for the demonstration of Mycobacterium leprae is the:
A. Fite
B. PAS
C. Kinyoun
D. Gram
A. Fite
The PAS reaction will demonstrate fungi, because the cell wall contains:
A. Lipids
B. Carbohydrates
C. Reducing substances
D. Argyrophilic protein
B. Carbohydrates
A stain that may be used to demonstrate calcium is the:
A. Gomori chromium hematoxylin
B. Fontana-Masson
C. von Kossa silver nitrate
D. Schmorl method
C. von Kossa silver nitrate
The Perl’s stain is used for the detection of:
A. Reducing substances
B. Hemosiderin
C. Hemoglobin
D. Calcium
B. Hemosiderin
A tissue frequently used as a control for the Fontana-Masson stain is:
A. Skin
B. Lymph node
C. Liver
D. Spleen
A. Skin
Toluidine blue stains:
A. Helicobacter
B. Legionella
C. Spirochetes
D. Chlamydia
A. Helicobacter
Acridine orange demonstrates:
A. Cell wall
B. Mitochondria
C. Nucleic acid
D. Flagella
C. Nucleic acid
Spirochetes in fixed tissue are best demonstrated by:
A. Histochemical techniques
B. Vital staining
C. Metallic impregnation
D. Physical methods
C. Metallic impregnation
Muscle that histologically contains cytoplasmic cross-striations and has multiple nuclei located at the edge of the fibers is classified as:
A. Smooth
B. Visceral
C. Skeletal
D. Cardiac
C. Skeletal
The terms “squamous,” “cuboidal,” and “columnar” describe cells that have their origin in which tissue?
A. Connective
B. Muscle
C. Epithelium
D. Bone
C. Epithelium
Another name for fat cells is:
A. APUD cells
B. Myocytes
C. Adipocytes
D. Histiocytes
C. Adipocytes
Elastic fibers have an affinity for:
A. Sudan
B. Indigo
C. Brazilin
D. Orcein
D. Orcein
Transitional epithelium refers to:
A. Endothelium
B. Urothelium
C. Mesothelium
D. Metaplasia
B. Urothelium
Which of the following histological features is unique to cardiac muscle?
A. Cross-striations
B. Peripherally located nuclei
C. Intercalated discs
D. Non-branching fibers
C. Intercalated discs
In addition to its ability to stain argentaffin cell granules, the Fontana-Masson technique may also be used to stain:
A. Lipids
B. Collagen
C. Melanin
D. Spirochetes
C. Melanin
A delicate three-dimensional connective tissue meshwork that forms the framework of organs, such as the spleen and lymph nodes, is made of:
A. Elastic fibers
B. Reticular fibers
C. Smooth muscle
D. Basement membrane
B. Reticular fibers
The connective tissue cells actively involved in wound healing are:
A. Plasma cells
B. Mast cells
C. Macrophages
D. Fibroblasts
D. Fibroblasts
An example of an exogenous pigment is:
A. Argentaffin
B. Melanin
C. Chromaffin
D. Carbon
D. Carbon
The staining method considered to be most sensitive and specific for COPPER is the:
A. Chloranilic acid
B. Rhodanine
C. Orcein
D. Aldehyde fuchsin
B. Rhodanine
The nerve process carrying electrical impulses away from the cell body is called a(n):
A. Neuron
B. Dendrite
C. Synapse
D. Axon
D. Axon
Dendrites bring electrical signals to the cell body and axons take information away from the cell body.
Hemosiderin, hemoglobin, and bile pigment are classified as:
A. Endogenous pigments
B. Artifact pigments
C. Exogenous pigments
D. Extraneous pigments
A. Endogenous pigments
Malignant tumors of connective tissue are known as:
A. Carcinomas
B. Lipomas
C. Sarcomas
D. Fibromas
C. Sarcomas
A rhabdomyosarcoma (malignant tumor, skeletal muscle) is suspected in a biopsy submitted to the laboratory. To aid in making a definitive diagnosis, a helpful stain would be the:
A. Phosphotungstic acid-hematoxylin (PTAH)
B. Gomori aldehyde fuchsin
C. Verhoeff-van Gieson
D. Periodic acid Schiff
A. Phosphotungstic acid-hematoxylin (PTAH)
The use of mounting media makes stained tissue components more visible by:
A. Distinguishing only the nuclei
B. Distinguishing only the cytoplasm
C. Destaining after hematoxylin
D. Improving the index of refraction
D. Improving the index of refraction
Neuritic plaques of Alzheimer’s disease consist of abnormal cell processes often in close proximity to deposits of:
A. Amyloid
B. Phospholipids
C. Neuromelanin
D. Astrocytes
A. Amyloid
A disorder involving excess iron deposition in tissues, with resultant TISSUE DAMAGE, is known as:
A. Hemosiderosis
B. Hemoglobinemia
C. Hemophilia
D. Hemochromatosis
D. Hemochromatosis
