HMP - ethical legal and logistical barriers

Question 1

problems obtaining metadata

Answer 1

host and environmental data from diverse backgrounds - culture, ethnicity and socioeconomic

Question 2

difficult looking at degree of genetic relatedness between the samples of twins

Answer 2

mono zygotic and dizygotis

-analysing the genetic factors v the environmental

Question 3

HMP plans

Answer 3

3000 reference genomes from humans

sequence to a high quality draft stage

Question 4

currently VBNC so we need to

Answer 4

viable non culture

improve culture methods and genome sequencing - in absence of culture

Question 5

normal microbiota - nasopharynx

Answer 5

S.aureus (+ve)

Question 6

normal microbiota - esophagus

Answer 6

streptococcus - +ve

Question 7

normal microbiota stomach

Answer 7

Helicobacter Pylori
gram -ve
e proteobateria

Question 8

normal microbiota small intestine

Answer 8

gram +ve rods and cocci, lactobacilli, streptococcus, enterococcus faecallis

Question 9

normal microbiota colon

Answer 9

gram +ve anaerobes - lactobacillus sp, chlostridium (spore forming)
gram -ve rods - klebsiella (Y proteobacteria)

Question 10

normal microbiota eye

Answer 10

S. aureus and S.epidermis

Question 11

normal microbiota ear

Answer 11

aspergillus, candida