HMP

Question 1

Pentose Phosphate pathway

• ______ is produced
• _______ is regenerated
• 6ribulose become _________

Answer 1

nadph

glucose

5glucose 6 phosphate

Question 2

Let’s go!!!

STEP 1

• _________ to ________
• ________ enzyme
• ______ is produced

STEP2

• ________ to _______
• __________ enzyme

Answer 2

glucose 6 phosphate to 6 phospho glucono lactone

glucose 6 phosphate dehydrogenase

6nadph

6 phosphoglucono lactone to 6 phospho gluconate; gluconolactone hydrolase

Question 3

Let’s keep going

STEP3
________ to _______
-______ is produced

______ to ———-
-_________ enzyme

STEP4

_________ is ______ to _______ or ______ to _________
-________,_____ enzymes

Answer 3

6 phosphogluconate acid to 6 keto phosphogluconate

6nadph

6 keto phosphogluconate to ribulose 5 phosphate

6 phosphogluconate dehydrogenase

ribulose 5 phosphate; isomerized; ribose5phosphate; epimerized; xylulose5phosphate

isomerase, epimerase

Question 4

Almost there

STEP5

Transfer of __C from ______ to _____ to give ________ and ______
-________

STEP6

Transfer for __C from _____ to _____ to give _________ and __________
-________

Answer 4

2

xylulose5phosphate to ribose5phosphate

sedoheptulose7 phosphate and G3P

transketolase

3

sedoheptulose to G3P

fructose 6 phosphate and erythrose 4 phosphate

transaldolase

Question 5

Last one bitch!!!!

STEP7
Transfer of __C from ________ to ______ to give ______ and _____
-_______

STEP8
G3P is then converted to ____ and then ___

-reversal of _____

Answer 5

2

xylulose 5 phosphate to eryhthrose4phosphate

G3P and fructose 6 phosphate

transketolase

F6P; G6P

glycolysis

Question 6

HMP is also known as

“__________ pathway”

“____________ pathway”

“_______ pathway”

“_________ pathway”.

Answer 6

pentose phosphate

Dickens-Horecker

Shunt

Phosphogluconate oxidative

Question 7

Instead of ___ going through the ___ pathway, it is shunted through this HM pathway; so it is known as the shunt pathway.

Answer 7

glucose

glycolytic

Question 8

In the glycolysis there are a few bisphosphate intermediates; but in this pathway, there are ______ only; hence this is called hexose monophosphate (HMP) pathway.

Answer 8

monophosphates

Question 9

Since the first carbon atom of glucose is liberated as ____, it is called the direct oxidative pathway of glucose metabolism.

Answer 9

CO2

Question 10

About __% of glucose molecules per day are entering in this pathway.

The liver and RBC metabolise about __% of glucose by this pathway. The major purpose of this pathway is generation of reduced ______ and ___________ for nucleotide synthesis

Answer 10

10

30

NADPH

pentose phosphates

Question 11

The rate-limiting step of the HMP is ???

Answer 11

Converting glucose 6 phosphate to 6 phosphoglucono lactone

By glucose 6 phosphate dehydrogenase

Question 12

HMP is (inhibited or stimulated?) by NADPH

Answer 12

Inhibited

Question 13

Metabolic Role of NADPH formed by HMP Shunt Pathway

1. Required for reductive biosynthesis, such as _______,____,_______
2. Free radical scavenging
3. _________ integrity
4. Prevention of formation of ________
5. Detoxification
6. Preserving transparency of ____ of ____
7. Bactericidal activity of ____
8. Production of ribose and deoxyribose for ____ and _____ synthesis

Answer 13

fatty acid, cholesterol and steroids

RBC membrane

met-hemoglobin

lens of eye

macrophages

DNA and RNA