Histology Workshop

Question 1

Once centrifuged, the blood is allowed to stand for an hour, what is the top layer of the blood?

Answer 1

Plasma

Question 2

Once centrifuged, the blood is allowed to stand for an hour, what is the bottom layer composed of?

Answer 2

Cells (buffy coat(white cells and platelets) and packed red blood cells)

Question 3

Which is the most common blood cell?

• Red blood cells
• Eosinophils
• Platelets
• Neutrophils
• Lymphocytes

Answer 3

red blood cells

Question 4

What is the most common type of white blood cell?

Answer 4

Neutrophils

Question 5

Where is the cell are most of the acids?

Answer 5

The Nucleus

Question 6

Why is the nucleus often referred to as basophilic?

Answer 6

Because of it being acidic it as an affinity for the dye.

Question 7

Which blood cells don’t have a nucleus?

• Red cells
• Eosinophils
• Platelets
• Red cells and lymphocytes
• Red cells and platelets

Answer 7

Red cells and platelets

Question 8

What is the most common type of blood cell?

• Basophil
• Eosinophil
• Monocyte
• Lymphocyte
• Neutrophil

Answer 8

Neutrophil

Question 9

What is the second most common type of white blood cell?

• Basophil
• Eosinophil
• Monocyte
• Lymphocyte
• Neutrophil

Answer 9

Lymphocyte
( You cannot differentiate from a microscope whether a lymphocyte is a B cell or a T cell without chemical markers)
(Very few of your circulating cells are B lymphocytes, almost all are T lymphocytes).

Question 10

What do the white vacuoles within the cell indicate?

Answer 10

It indicates that the cell undergoes phagocytosis.

Question 11

What type of cell has a folded nucleus into a horse shoe/kidney bean shape and has no granules?

Answer 11

Monocyte

Question 12

What is the difference between monocytes circulating and once they have entered tissues?

Answer 12

Monocyte circulate in the blood and once they enter a tissue they become tissue specific.

Question 13

What white blood cell has a segmented nucleus with 2 lobes?

Answer 13

Eosinophil

Question 14

The machines in the lab are designed to analyse one cell at a time, what happens if a platelet clump goes through?

Answer 14

It will not register and so there will be a low platelet count because it is not designed to differentiate between multiple of the same cells.

Question 15

What is anaemia?

Answer 15

A lack of red blood cells often reflected in haemoglobin`

Question 16

How is red cell size expressed?

Answer 16

mean cellular volume (MCV)

Question 17

With anaemia, how can the red cell size can change?

Answer 17

Microcytic

Macrocytic or they can remain the same (normocytic)

Question 18

What is a protozoal infection caused by?

Answer 18

A parasite