Histology

Question 1

What is the most common fixative

Answer 1

formalin

Question 2

what is substituted to allow the processing of wax

Answer 2

xylene

Question 3

What is the most common staining technique and what do they stain

Answer 3

Haematoxylin and Eosin - H stains acids purple (basic) - E stains bases pink (acidic)

Question 4

What is massons trichrome

Answer 4

contains haematoxylin, acid fuschin and methyl blue

Question 5

what does massons trichrome stain?

Answer 5

stains collagen blue, muscle/cytoplasm red and nuclei black

Question 6

H and E does not stain for…

Answer 6

Mucin

Question 7

What does Periodic acid Schiff (PAS) stain for?

Answer 7

Mucin and Glycogen (also highlights fungi)

Question 8

Why is distase used before PAS staining?

Answer 8

to break down glycogen

Question 9

What stains for iron

Answer 9

Pearls prussian blue

Question 10

What does Orcein stain for?

Answer 10

stains for elastic fibres, Hep B and copper

Question 11

What can be used to stain fungi?

Answer 11

PASD or Crocott

Question 12

Ziehl Neelson stains for?

Answer 12

Mycobacteria

Question 13

What do cells in an active cell cycle produce

Answer 13

Ki 67