Histology Flashcards
What are the steps of preparing a sample?
10% buffered formalin to crosslink and fix, embed in parafin, sectioning on microtome, mount onto glass slides, stain and clear (tome means “a cutting)
How can you get the ultrastructure?
electron microscopy
Hematoxylin and eosin
hematoxylin causes blue nuclei, and eosin is pink cytoplasm
what is in situ hybridization and immunohistochemistry
immunohisto- tagging with fluoro antibodies, or in situ tags nucleic acids
immunohistochemistry: direct vs indirect detection
direct is when the antibody has a fluorotag attached right to it, and indirect is when another antibody acts as the antigen to the primary.
What counts as surface epithelium?
skin, sweat glands, breast glands, urogenital tract, intestinal GI tract, resp system, digestive glands.
What is mesothelium? What type of epithelium is it?
Mesothelium lines our inner cavities, like the peritoneal space (where the lining is called the perineum) and the thoracic space where the lining is the pleura
- simple squamous
- stratified squamous
- simple cuboidal
- stratified cuboidal
- simple columnar
- stratified columnar
- pseudostratified columnar
8.transitional
- mesothelium, endothelium, kidney glomeruli
- esophagus, vagina, skin
- kidney tubules
- some sweat glands
- stomach, digestive tract
- vas deferens, trachea
- lung
- bladder
cytokeratin 7
intermediate filament that provides cellular shape andstrucutre, in kidney
p63
a type of protein found in bronchi, involved in maturation of stem cells
what is the key cellular feature of a gland?
vacuolated spaces
What is myoepithelium?
surrounds glands, allows to contract and expel stuff.
