Herpes Simplex Viruses Flashcards
structure of HSV-1/2
type of genome
large(150nm)
enveloped
icosahedral
linear dsDNA - unique UL, US stretches(long and short) flanked by inverted repeats
where does HSV1/2 replicate?
where does assembly occur?
both assembly and replication occur IN the nucleus
how are HSV1/2 transmitted
HSV-1; oral/nasal; (rarely sexual)
HSV-2; genital
Disease caused by:
HSV-1
HSV-2
HSV-1
- gingivostomatitits
- herpes labialis(cold sores)
- keratoconjunctivitis
- encephalitis
HSV-2
- genital herpes
- neonatl herpes
- aseptic meningitis
which neural tissues are preferred by HSV1/2 for latency
DRG - primarily HSV-2
Trigeminal - primarily HSV-1
IE proteins in HSV are activated by what protein contained within the tegument of the original virus?
VP16
allows IE proteins to have transcription activated right away
no wait for proteins to be formed
areas most commonly affected by HSV-1 vs HSV-2
describe clinical manifestation/visual appearance
clear vesicle on erythematous base progressing to pustular lesions, ulcers
HSV-1 = facial
HSV-2 = genital
how is neonatal HSV transmitted
describe the manifestation of neonate infection
acquired by passing through birth canal
usually severe disease that extends to CNS/other organs
describe mechanism of latency in HSV infections
nucleocapsids transport to regional ganglia by retrograde axonal transport
VIral DNA is retained in sensory neurons, only expressing LAT gene
reactivation can occur w/virus transporation back down axon
ELVIS
enzyme linked virus inducible system
genetically engineered cell line
HSV are plated and when they grow in these cells, galactosidase is produce, staining blue;
used for early HSV detection
HSV DNA in CSF is diagnostic of….
herpes simplex encephalopathy
tissues affected by HSV
skin, cornea, CNS
