Hemoglobin Flashcards
Cyanmethemogmobin is measured at
540 nm
converts the hemoglobin into methemoglobin
Potassium ferricyanide
Full conversion of hemoglobin to cyanmethemoglobin
10 minutes at RT
Another technique that has been used in some automated instruments involves the use of
Sodium lauryl sulfate
an example of commercially available handheld system to measure the hemoglobin concentration
HemoCue
Movement of charged particles in an electrical field
Electrophoresis
fastest normal Hb
Hemoglobin A1
fast hemoglobin migrate beyond A1
Bart Hb
Hb H
Hb I
Slowest abnormal Hb
Hb A2
Hb C
Hb E
Hb O
Hb C harlem
Site of heme synthesis
Mitochondrion
heme biosynthesis is most prominent in
BM and Liver
enzyme needed to insert the Fe2+ to the P IX ring
Ferrocheletase (Heme synthetase)
predominant form of Hb in the fetus and newborn
Hemoglobin F
Predominant form of Adult hb
Hemoglobin A1
hb w/ Fe2+ bound to Carbon Monoxide, 240x greater affinity to Hb than O2
Carboxyhemoglobin