Hema 2 Lab M2 Flashcards
Small, round, or oval, anucleate cytoplasmic fragments of megakaryocytes
Platelets (Thrombocytes)
Most accurate way of platelet count
Reese-Ecker
True blood cells and produced in the bone marrrow
Platelets (Thrombocytes)
Maturation period of Thrombocytes
5 Days
Lifespan of Thrombocytes
8-11 days
Anticoagulant used in Platelet count
EDTA
Immediate precursor of Thrombocytes
Megakaryocytes
Precursor of Megakaryoctes
Megakaryoblasts
Functions of the Thrombocytes
Prevents bleeding
Maintains homeostasis
Wound healing
What are the factors released by the thrombocytes?
Fibrinogen
Thrombin
Prothrombin
Also known as thrombocytes
Platelets
Recommended method for manual platelet count
Brecher-Conkrite method
Purpose of the Reese-Ecker fluid
Preserve and stain the thrombocytes
Type of pipette used in the Reese-Ecker method
RBC pipette
How many large central squares are counted in the Reese-Ecker method?
25 large central squares
Reference range of platelet count
150,000-450,000/ uL
150- 450 x 109/ L
Refers to the adherence of platelets around neutrophils, producing a ring or satellite effect and occurs when an EDTA anticoagulant is used
Platelet satellitism
Decreased number of circulating platelets
Thrombocytopenia
Abnormally high platelet count, typically 450,000/uL
Thrombocytosis
What is the color of thrombocytes?
Gray-blue with purple granules
Reagents in Guy and Leakeβs Method
40% formaldehyde (HCHO), sodium citrate, crystal violet
TRUE or FALSE
Platelets must be counted within 2 hours if venous blood is used.
FALSE
dapat 3 hours
Advantages of Automated platelet counting
- Speed of performance
- Elimination of visual fatigue of the technician
- Improved precision
- Reduction in pre-analytic and post-analytic variables
- Corrects identification of sample for processing and analysis, storage, and retrieval
graphic representation of a collection of data based on cell size and/or cell number decipting variations in the process.
Histogram
Histogram is also called ______
frequency of distribution curve
x axis
cell volume cell
y axis
cell number
platelets with a size of 8-12 fL are counted between ______
2 and 25 fL
Can be counted as platelets, hence an increase in PLT and decrease in RBC
Microcytes & schistocytes
Ideal microscope used for platelet count
Phase contrast microscope
Ratio of platelets : RBC
1:20
What is the platelet value of thrombocytopenia?
lower than 100,000/uL
Refers to the adherence of platelets around neutrophils,
producing a ring or satellite effect
Platelet satellitism
What anticoagulant will use to correct platelet satellitism?
sodium citrate
TRUE or FALSE
Skin puncture specimen is less desirable because of the
tendency of the platelets to aggregate or form clumps.
TRUE
100,000 - 149,000/ul
Slight decrease
Formula for Platelet Estimate
Formula:
π·ππππππππ/ππ³ = π΅π. ππ π·ππππππππ πͺππππππ
π ππ, πππ
In the modified Indirect Method, the smear will be stained by ________.
Wrightβs or Giemsa stain
What type of Indirect method uses 14% Mg as a reagent?
Fonioβs Method
In the modified indirect method, how many fields will we count?
10 consecutive
OIO fields
How many drops should we expel after shaking/distribution of specimen and diluting fluid.
4-5 drops
Diagnose bleeding disorders and monitor conditions affecting BM
Platelet count
Estimate of platelets per field
8-20
