Heat Flashcards
What does heat do to microbes?
Kills microbes and denatures proteins
What does cold do to microbes?
Inhibit microbial growth
What is the minimum growth temperature?
Lowest temp an organism will grow
What is the optimal growth temperature?
Where the organism grows the fastest
What is the maximum growth temperature?
Highest temperature the organism will grow
What is the temperature classification of a psychrophile?
15’ or less
What is the temperature classification of psychotrophs?
15’ - 25’
What is the optimal temperature of mesofiles?
25’- 45’
What is the optimal temperature of thermofiles?
45’-65’
What is the optimal temperature for hyperthermophiles?
About 80’ or higher
What are the two types of dry heat?
Incineration and hot air ovens
What are the standard parameters of hot air ovens?
171’ for 1 hour or 160’ for 2 hours
What are the four types of moist heat?
Pasteurization,boiling, Tyndallization, and autoclave
What are the standard parameters of pasteurization?
63’ for 30 minutes
72’ for 15 seconds
140’ for 3 seconds
What are the standard parameters of boiling?
100’ for 15 minutes
What are the standard parameters of tyndallization?
Boil 100’ for 30 minutes and incubate at 37’ overnight
What are the standard parameters of autoclaving?
121’ for 15 minutes, add 15 psi of pressure
What is disinfection?
Kills vegetative bodies only
What is sterilization?
Kills vegetative bodies and endospores
