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Microbiology Lab Practicum 1 > Heat > Flashcards

Heat Flashcards

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Biology 101 flashcards
1
Q

What does heat do to microbes?

A

Kills microbes and denatures proteins

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2
Q

What does cold do to microbes?

A

Inhibit microbial growth

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3
Q

What is the minimum growth temperature?

A

Lowest temp an organism will grow

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4
Q

What is the optimal growth temperature?

A

Where the organism grows the fastest

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5
Q

What is the maximum growth temperature?

A

Highest temperature the organism will grow

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6
Q

What is the temperature classification of a psychrophile?

A

15’ or less

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7
Q

What is the temperature classification of psychotrophs?

A

15’ - 25’

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8
Q

What is the optimal temperature of mesofiles?

A

25’- 45’

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9
Q

What is the optimal temperature of thermofiles?

A

45’-65’

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10
Q

What is the optimal temperature for hyperthermophiles?

A

About 80’ or higher

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11
Q

What are the two types of dry heat?

A

Incineration and hot air ovens

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12
Q

What are the standard parameters of hot air ovens?

A

171’ for 1 hour or 160’ for 2 hours

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13
Q

What are the four types of moist heat?

A

Pasteurization,boiling, Tyndallization, and autoclave

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14
Q

What are the standard parameters of pasteurization?

A

63’ for 30 minutes
72’ for 15 seconds
140’ for 3 seconds

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15
Q

What are the standard parameters of boiling?

A

100’ for 15 minutes

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16
Q

What are the standard parameters of tyndallization?

A

Boil 100’ for 30 minutes and incubate at 37’ overnight

17
Q

What are the standard parameters of autoclaving?

A

121’ for 15 minutes, add 15 psi of pressure

18
Q

What is disinfection?

A

Kills vegetative bodies only

19
Q

What is sterilization?

A

Kills vegetative bodies and endospores

Microbiology Lab Practicum 1 (15 decks)

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