guia 11-2

Question 1

caracteristica: tampon biologico funcion: mentiene estable Ph (7-8)

Answer 1

TRIS (hidroximetil amino metanol)

Question 2

caracteristica: agente quelante funcion: toma iones de Mg

Answer 2

EDTA

Question 3

caracteristica: sal funcion: precipita el ADN

Answer 3

Acetato de sodio

Question 4

caracteristica: sal funcion: precipita proteinas

Answer 4

acetato de potasio

Question 5

caracteristica: solvente organico funcion: desnaturaliza proteinas

Answer 5

Fenol

Question 6

caracteristica: solvente organico funcion: remueve lipidos y solubiliza el fenol

Answer 6

cloroformo

Question 7

caracteristica: detergente anionico funcion: solubiliza proteinas, tej y membranas

Answer 7

SDS

Question 8

caracteristica: detergente aionico funcion: inhibe hexoquinasa

Answer 8

Sarkosyl

Question 9

caracteristica: Dtergente cationico funcion: solubiliza polisacaridos

Answer 9

CTAB

Question 10

caracteristica: detergente polar funcion: disuelve componente de tejidos y membranas

Answer 10

triton x-100

Question 11

caracteristica: Antioxidante funcion: escinde puentes disulfuro y evita el color pardo

Answer 11

B mercaptoetanol

Question 12

caracteristica: antioxidante funcion:reduce grupos sulfuros de proteinas

Answer 12

DTT

Question 13

caracteristica: Alcohol funcion: disminuye formacion de interfases

Answer 13

alcohol isoamilico

Question 14

caracteristica: Detergente funcion:elimina elementos fenolicos

Answer 14

PVP

Question 15

caracteristica: alcohol funcion: precipitacion de acidos nucleicos

Answer 15

ETanol

Question 21

caracteristica: Enzima funcion:elimina RNA

Answer 21

RNAasa

Question 21

caracteristica:sales funcion:equilibra fuerzas ionicas

Answer 21

NACL, KCL

Question 21

caracteristica: agitador funcion: desproteinacion de ADN

Answer 21

agitador de vortex