Growth control

Question 1

Decontamination?

Answer 1

Wiping

Question 2

Sterilization?

Answer 2

Remove all microorganisms

Question 3

Disinfection?

Answer 3

Agents that kill or inhibits growth

Question 4

Sterilants?

Answer 4

Sterile –> Destroy all microorganisms + endospores

Question 5

Disinfectants?

Answer 5

Kill microorganisms but not endospores

Question 6

Sanitizers?

Answer 6

Reduce the number but do not kill

Question 7

Antisepticts?

Answer 7

Kill or inhibits growth

Question 8

3 antimicrobial controls?

Answer 8

H
R
F

Question 9

Heat?

Answer 9

Measured in D = 10fold reduction in viability

Moist heat has better penetration @ given T

Question 10

Thermal death time?

Answer 10

Basically the t for all cells to be killed. Sample heated –> medium –> incubated

Question 11

Autoclavation?

Answer 11

Sterilization with steam under P @ high T a smaller sample - less t large sample - more t

Question 12

Pasterurization?

Answer 12

Do not kill endospores
Reduces the nr of midroorganisms in food and increases the shelf life.
Flashpasteruization is a developed method

Question 13

Radiation?

Answer 13

UV - for disinfection

Question 14

Ionization?

Answer 14

Basically energy that penetrates and kills.

Have difficulty killing endospores, virus and resistant microorganisms.

Question 15

Filter?

Answer 15

Used for not T sensitive and and no ionization. Pores that are 0.2um but cannot catch viruses.

Question 16

Depth?

Answer 16

Sheets of glassfiber
HEPA
Pores >0.3um that removes from air

Question 17

Membranes?

Answer 17

Used in labs. Small polymers with a high number of small pores.
Syringe/pump for (l) to a sterile vessel

Question 18

Nucleopore?

Answer 18

Polycarbonate film + radiation + chemicals that isolate specimens for scanning in electron microscope.

Question 19

Bacteriocidal agents?

Answer 19

Kills but do not bind and lyse

Question 20

Bacteriostatic agents?

Answer 20

Inhibits and binds weak

Question 21

Bacteriolytic agents?

Answer 21

Kills by binding and lysing

Question 22

5 basic mechanisms for agents?

Answer 22

Inhibit cellwall synthesis
Inhibits translation 
Alternates the cell membrane
Inhibits nucleic acid synthesis
Antimetabolite activity

Question 23

MIC?

Answer 23

Minimum inhibatory concentration

Smallest amount that completely inhibits growth

Question 24

E-test?

Answer 24

Plastic strip + [] of microbial agent to agar platform. From plate to agar and forms an eliptical zone of inhibition where the eclipse meets the strips mic.

Question 25

Kirby-Bauer test?

Answer 25

Disc diffusion test for sensitivity or resistance of aerobes and anaerobes. Produces an inhibatory zone = no growth and the lowest [] with longest D into the media has the highest sensitivity to the bacteria.

Question 26

Zone of inhibition?

Answer 26

It is compared to a chart: 
Sensitive 
Resistant 
Intermediate 
Basically zone of I + MIC value