Growing Viruses Flashcards
what is the most common method of isolating a virus
cell culture
what are the purposes of viral amplification
diagnostic purposes
research
vaccine production
where can the growing of a virus take place
cell culture
embryonated eggs
neonate mice
other lab animals
what are the types of cell cultures (3)
primary cells
semi-continuous cells
continuous cells
which type of cell culture has cells harvested form fresh tissues
primary cells
which type of cell culture has cells from human embryonic kidney and skin fibroblasts
semi-continuous cells
which type of cell culture are usually transformed and immortal
continuous cell lines
what is the gold standard in finding new viruses
virus isolation in cell culture
how can vaccines be done nowadays
recombinant DNA technology
what is “changes in the infected cells such as ballooning of cells or syncytia formation”
cytopathic effect
what are 2 things that may occur to growing viruses in cell cultures
cytopathic effect
hemadsorption
what is hemadsorption
cells acquiring the ability to stick to mammalian red blood cells
true or false:
a 4 fold or more increase in a virus-specific titer of IgG or total antibody between acute and convalescent sera can determine a primary infection
true
true or false:
a single high titer of IgG (or total antibody) is a confirmatory test for disease
false
it only confirms previous exposure not active infection
what is the criteria for diagnosing reinfection
1 fold or more increase in titer of IgG or total antibody between acute and convalescent sera
absence or slight increase in IgM
