Gram Staining/Antibiotics [B]

Question 1

How do antibiotics get rid of cells?

Answer 1

They target the cell wall and destroy it

Question 2

What is the difference between Gram positive and Gram negative cells?

Answer 2

Antibiotics can destroy gram positive cells but can’t destroy gram negative
Gram positive cell only has a thick cell wall
Gram negative has a thin cell wall but a protective layer

Question 3

Why can’t singular antibiotics destroy gram negative cells?

Answer 3

The protective layer prevents antibiotics from reaching the cell wall

Question 4

What is the Gram Staining method used for?

Answer 4

To identify which cell is gram positive and which is gram negative

Question 5

What are the steps to gram staining?

Answer 5

Get heat fixed cells, Apply crystal violet dye, Apply iodine solution, Wash with alcohol, Counter Stain with Safranin

Question 6

What are the results for each cells after gram staining?

Answer 6

Gram positive cells will remain purple throughout the staining
Gram negative will lose the purple colour at alcohol washing and will be red/pink at the end