Gram stain Flashcards
1
Q
what is the gram stain
A
differential stain based on difference of cell walls
2
Q
what is the first step
A
primary stain, purple basic dye. all cells stained
3
Q
what is the second step
A
mordant is iodine sln, formals a large complex w/primary stain
4
Q
what is the third step
A
alochol decolourizer, rmoves iodine complex from gram -ive cell wall
5
Q
what is the fourth step
A
counter stain, stain is pink colour basic dye, results gram -ive are pink gram +ive are violet
