Gram Negative Rods GIT GUT Flashcards
Characteristic appearance of E. coli in EMB?
Metallic green sheen
Special mediums used for gram negative enterics?
EMB
MacConkey
Lactose vs Non lactose fermenters
Lactose fermenters?
maCconKEE Citrobacter Kleb Enterobacter E. coli
Habitat of E. coli?
Human colon
Colonize vagina and urethra
Most common cause of community acquired UTI in the Philippines (underdeveloped countries)?
Also the most common cause of gram negative sepsis?
E. coli
Transmission of E. coli is via?
UTI - ascending
Neonatal men - birth
Diarrhea - fecal oral
Top three causes of neonatal meningitis?
GEL
GBS
E. coli
Listeria
Which E. coli strain causes watery diarrhea also known as traveler’s diarrhea?
ETEC
T - Toxigenic - Travelers
What causes watery stools in travelers diarrhea?
Pili + HST and HLT (cholera-like)
Inhibit Na reabsorption
Increase Cl and HCO3 secretion
TX: Hydrate!!
Which E. coli strain causes bloody diarrhea?
EHEC
H - Hemorrhagic - HUS
EHEC strain that causes HUS?
Anemia. Thrombocytopenia. Renal failure
E. coli O157:H7
Virulence factor responsible for bloody diarrhea and HUS?
Shiga like verotoxin
Inhibit 60s - no protein synthesis - cell death
Strain of E. coli that also causes bloody diarrhea due to invasion of epithelial cells with fever and CRAMPY abdominal pain?
EIEC
Invasive
Same toxin as shigella
Strain of E. coli that causes watery diarrhea of long duration in developing countries?
EPEC
P Pathogenic Pedia Philippines
Strain of E. coli that cause watery diarrhea in the immunocompromised?
EAEC
A Adherent AIDS
DOC for E. coli meningitis and sepsis? UTI?
Men and sepsis - Ceftriaxone
UTI - Ampicillin
Top three causes of gram negative sepsis
EK
E. coli
Kleb
Urease positive, with ESBL that cause nosocomial pneumonia in alcoholics?
Klebsiella pneumoniae
Characteristic sputum of Kleb pneumonia?
Currant jelly sputum
Intussusception
Antigens present in Kleb?
Only K antigen (Kapsule)
No H antigen - nonmotile
Gram negative enteric that causes rice water stools?
Vibrio cholerae
Toxin responsible for watery stools in cholera?
Choleragen - ADP ribosylation
Inhibit Na reabsorption
Increase Cl and HCO3 secretion
Characteristic shape and motility of Vibrio cholerae?
Comma shaped
Shooting star motility
(Trichomonas falling leaf, Proteus swarming, Listeria tumbling)
What special medium is used to culture Vibrio cholerae?
TCBS
Thiosulfate Citrate Bile salts Sucrose
Habitat of Vibrio cholerae?
Colon
Transmitte fecal oral by ingestion of fecal contaminated water
Species of Vibrio in raw seafood and in seafood handlers?
Raw - V. parahemolyticus
Handlers - V. vulnificus
DOC for cholera?
Tetracycline
Fluid replacement
DOC for v. parahemolyticus and vulnificans?
Minocycline
FQ
MCC of bacterial diarrhea overall?
Campylobacter jejuni
MCC of adult diarrhea? Pedia diarrhea?
Adult - Norwalk virus
Pedia - Rotavirus