Gram Negative Flashcards
Moraxella
Mucous membranes
Paired coccoid rods
capsule, weak
Blood agar
2 subgenera:
Moraxella
- M. Lacunata: human keratoconjunctivitis
- M. Bovis: fimbria, B-haemolysis. cattle infectious keratoconjunctivitis
Branhamella
B. Ovis: ru keratoconjunctivitis
Brucella
Genitals mucous membranes Can't survive in environment Coccoid rods Obligate AEROBE blood agar, CO2 (abortus/ovis), R-S colonies KÖSTER staining (modified Ziehl-Neelsen) Susceptible to acidic PH UREASE, oxidase, catalase positive Facultative IC Tbilisi phage Susceptible to detergents,heat,PH
Abortion, epididimitis , arthritis
- B. Melitensis: sheep+goat - orchitis
- B. Abortus; Ru, Hu
- B. Suis, canis, ovis , microti
Taxonomy: monosp.: b. Melitensis biovar abortus 1,2,3 // multisp. B. Abortus 1,2,3
Brucella colonies
S - capsule , R - non- capsulated
R type: B. Ovis, B. Canis - cross reaction
S type: B. Melitensis, B abortus , B. Suis cross reaction
Bordetella
Respiratory mucous membranes
Rod
Fimbria
Obligate aerobic
C+O +
Toxin production
1. B. Pertussis: hu whopping cough, aerosol, adhere
2. B. Parapertissis: hu parapertissis, ovine pneumonia
3. B. Bronchiseptica : (dermonecrotoxin - osteoblasts, tracheotoxin- epithelial). Media contain only citrate, O,K,H,F Ag.
Facultatively pathogen- su- atrophic rhinitis, Dog- Distemper!!! , Cat: bronchitis
4. B. Avium: capsule , cyto+dermonecrotoxin , Coryza-like disease , avian bordetellosis
Francisella
Rodents, arthropods - facultative IC Rabbit is the main reservoir (tick- vector) Thin capsule Coccoid rod Facultative IC Weak , good in cold Bipolar staining Blood agar/ CA+ sulphur containing aa C+ , O- Bioweapon
F. Tularensis ssp. Tularensis + F. Tularensis ssp. Holarctica - hu+ animals: Tularaemia (granuloma, necrosis)
*Holarctica: not in farm animals
Taylorella
Genital mucous membrane of Stallion, clitoris in mare Fimbria Small coccoid rod Capsule Chocolate agar , CO2 C+ O+
T. Equigenitalis: CEM -contagious equine metrits
Riemerella
BIRDS mucous membranes Rod Blood agar + CO2 Grey, mucoid , non- haemolytic colonies O+ , C+
R. Antipastifer: duck , goose, turkey : antipastifer disease: ocular and nasal discharge, meningitis, fibrous peritonitis, pericarditis (up to 6w age)
Ornithobacterium
BIRDS upper respiratory tract Polymorphic Bipolar staining Blood agar+ CO2 Non-haemolytic C- O+ Not zoonotic
O. Rhinotacheale: facultative pathogen trachaeitis
Haemophilus+ Avibacterium
Respiratory + genital mucous membranes Rod cocciod Filaments, capsule , fimbria, flagella Weak Facultative anaerobic Blood/chocolate agar X +V factor Cell wall+ capsule Ag
Hemophilus
- H. Influenza: upper hu respiratory way , meningitis, aerosol
- H. Parasuis: CO2 , V factor, su: polyseroslits (Glasser’s disease)
Avibacterium:
A. Paragallinarium: infectious coryza ,CO2+ V factor
A. Gallinarum (resp.) , An avium (V, resp)
Histophilus
Urogenital+ respiratory mucousa
Chocolate agar , CO2
Yellowish colonies
Facultative pathogen
H. Somni: Bo: spectemia, TEME: Thronboembolic meningoencephalitis.
Sheep: orchitis, abortion, mastitis
Actinobacillus
Mucous membranes
Animal carriers , doesn’t survive long in the environment
facultative anaerobic
blood agar
6 species:
1. A. Lignieresii: Ru oral cavity+rumen , rod , filaments , sulphur granules , bipolar staining , , (no haemolysis) , C+ , O+
Ru+su Actinobacillosis (lesions tongue, skin, rumen)
2. A. equuli: eq+pig oral cavity , intestine
Rods,filaments, bipolar staining, large mucoid colonies C+,O+
Sleepy foal disease, eq: pyospecaemia , su: spectemia
3. A. suis: swine tonsilla, mucous memb., Haemolysis.
Piglets+foals: spectaemia+ pneumonia
4. A. Seminis: genitalia, semen, CO2 (No Haemolysis) ram: epididimitis , lamb: polyarthritis , sheep: abortion
5. A. Ureae: hu respiratory infection
6. A. Pleuropneumoniae: su pleuropneumonia
Fimbria, capsule, BA/CHA (haemolysis)
Biotype 1: V factor , biotype 2: not
Mannheimia + Bibersteina
Mucous membrabnes, Ca oral cavity (dog bite) coccoid rods Bipolar staining , Giemsa Blood agar , B haemolysis O+ C+ Facultative anaerob Cytotoxin production Capsule Fimbria
- Mannheimia haemolytica: bovine pneumonic pasterurelosis (shipping fever) , pneumonia+mastotis in sheep
- Bibersteina trehalosi: sheep+ goat acute pasturelosis
Pasturella
Mucous membrabnes, Ca oral cavity (dog bite) coccoid rods Bipolar staining , Giemsa Blood agar , B haemolysis O+ C+ Facultative anaerob Cytotoxin production Capsule Fimbria
- P. Multocola: grey colonies , sweetish odor
Types A,D: cattle+sheep: pneumonia, mastitis
Poultry: fowl cholera
Type B,C: cattle haemorragic spectaemia - P. Pneumotropica ,canis , dogmatis, stomatis: Ca oral cavity+ resp. Tract, bite wound abcesses
- P. Aerogenes: swine alimentary tract, abortion
Bacteriods + Dichelobacterae + Pervotella
GI+Resp+genital tract Rod Fimbria Obligate anaerobic Fastidious
Necrosis, abcesses
Bacteriodes:
- B. fragilis: hu gut flora-
- B. Ureolyticus: GT
Dichelobacter:
D. Nodosus: sheep foot rot (keratinase, protease)
Prevotella:
1. P. Melaninogenicus: Ca putrid inflammation
Fusobacterium
Polymorph Weak Fastidious Obligate anerobic CO2 Selective: crystal violet, brilliant green Inactive Facultative pathogen- anerobic wounds, abcesses
1.F. Necrophorum: filaments, blood agar, hyaluronidase
F. Necrophorum ssp. Necrophorum: sheep foot rot
2. F. Nucleatum: hu+ca: abcesses, peritonitis, mastitis
