Gram Indeterminate Bacteria

Question 1

Important characteristics of Chlamydia

Answer 1

Obligate intracellular
Cannot be gram stained
No muramic acid in cell wall
Can’t synthesise ATP (energy parasites)

Question 2

Forms of Chlamydia

Answer 2

Elementary body (infective form)
Reticulate body (replicative form) (binary fission)

Question 3

Ags in Chlamydia

Answer 3

LPS
Species specific protein Ag
Serovar specific Ag (MOMP)

Question 4

Stains for Chlamydia

Answer 4

Giemsa
Castadena
Lugols iodine (for inclusions)

Question 5

Culture of Chlamydia

Answer 5

Egg yolk

Cell line culture (HeLa, Hep2)

Question 6

Main method of DX of Chlamydia

Answer 6

NAAT

Question 7

Types of Chlamydia

Answer 7

C. Tranchomatis
C. Pneumoniae
C. Psittaci

Question 8

Serotypes of c. Tranchomatis and their diseases

Answer 8

A-C -> Blindness (keratoconjunctivitis)
D-K -> STI
L1-L3 -> lymphogranuloma venereum

Question 9

Blindness caused by C. Trachomatis

Answer 9

Leading cause of blindness in world

Transmission - hand to eye contact

Question 10

STI caused by C. Trachomatis

Answer 10

Water discharge
Complications: PID, cervicitis, salpingitis
Can transmit to baby

Question 11

Neonatal effects of STI caused by C. Trachomatis

Answer 11

Neonatal conjunctivitis (in 1-2 wks)
Neonatal pneumonia (stecotto cough)

Question 12

Lymphogranuloma venereum

Answer 12

C. Tranchomatis
Inguinal l.n
Painless genital ulcers progress to tender painful lymphadenopathy

Question 13

Reactive arthritis

Answer 13

Reiters syndrome
AI
Sacroiliac and knee
Uveitis, urethritis, arthritis (can’t see, can’t pee, can’t climb a tree)

Question 14

C. Pneumoniae

Answer 14

Atypical/walking pneumonia
Elderly
-Pharyngitis
-sinusitis
-Exacerbate asthma and COPD

Question 15

C. Psittaci

Answer 15

Pneumonia transmitted from birds

Question 16

Treatment of Chlamydia

Answer 16

No penicillins
Azithromycin
Doxycycline
Ceftriaxone (for gonorrhea coinfection)

Question 17

General features of coxiella burnetti

Answer 17

Gram -

Obligate intracellular

Question 18

Transmission of coxiella burnetti

Answer 18

Spore like structures found in animal feces

Aerosol transmission

Question 19

Coxiella burnetti C/F

Answer 19

Q fever:

• NO RASH
• HEPATITIS
• Fever
• Pneumonia
• Headache

Self limiting

Question 20

Gardnerella vaginalis causes

Answer 20

Bacterial vaginosis

Question 21

Gardnerella vaginalis general features

Answer 21

Gram variable rod
Non motile
Metachromatic granules