Good Microbiological Lab Practices Flashcards
Safety Rules and Regulations
What precaution should be taken regarding doors and windows during laboratory sessions?
Keep doors and windows closed to prevent contamination from air currents.
What should students do before and after each laboratory activity?
Students must wash their hands thoroughly with soap and water.
How should all microorganisms, especially unknown cultures, be treated in the microbiology laboratory?
Treat all microorganisms as if they were pathogenic.
What technique should be practiced to ensure only one type of microorganism is present in a pure culture?
Aseptic Technique.
What must be done to all materials needed for an experiment?
All materials must be sterilized.
What should be done with inoculating loops and needles before and after use?
Flame sterilize them.
What precautions should be taken when working with open flames?
Secure loose hair, clothing, jewelry, and nearby paper.
What should be done with heat sources when not in use?
Do not leave them unattended and keep flammable liquids at a safe distance from flames.
What should be avoided when working with chemicals or microorganisms?
Keep hands away from the face, eyes, and mouth.
How should liquid cultures be aspirated and dispensed?
Never pipette by mouth; use pipette bulbs or pipetting devices.
What precaution should be taken regarding glassware before using it?
Check for cracks and chips and inform the instructor if any are found.
