A. Practice Aseptic Technique Flashcards
What is the first step in aseptic technique when transferring microorganisms?
The first step is to sterilize the inoculating loop before and after each transfer.
What is an important point to remember when performing aseptic transfers with the inoculating loop?
It’s not necessary to see a mass of bacteria on the loop during a transfer; even a single cell is adequate to start a new culture.
What is the temperature at which the inoculated cultures should be incubated for 24 - 48 hours?
The cultures should be incubated at 37°C.
What should you do after incubating the inoculated cultures for the designated time period?
After incubation, observe whether the transfers were successful, and then discard the cultures.
Why is it necessary to sterilize the inoculating loop before and after each transfer in aseptic technique?
Sterilizing the loop helps prevent contamination of the cultures and ensures that only the desired microorganisms are transferred.
What are some key techniques for holding tubes, caps, and the inoculating loop during aseptic transfers?
Properly holding the tubes, caps, and loop helps minimize the risk of contamination. Techniques for holding these items will be demonstrated in class.
What should you do if you encounter difficulties or uncertainties while performing aseptic transfers?
You are encouraged to ask for assistance or a critique while performing the transfers to ensure proper technique and minimize errors.
What is the purpose of transferring microorganisms from a plate to an agar slant and vice versa?
Transferring microorganisms between these two media allows for different growth conditions and long-term storage of cultures.
Why is it important to observe the transferred cultures after incubation?
Observing the cultures allows you to assess whether the transfers were successful and whether the desired growth occurred.
What is the significance of incubating the cultures at 37°C specifically?
Incubating at 37°C mimics the optimal temperature for the growth of many human pathogens, making it suitable for culturing a wide range of microorganisms.
