Culture Media Preparation Flashcards
How do you prepare tryptic soy broth from commercially available dehydrated medium?
Suspend 30 g of the dehydrated medium in 1000 ml of distilled water, mix thoroughly in a 2 liter Erlenmeyer flask, and sterilize for 20 minutes at 121°C (15 lbs pressure).
How do you prepare media from a formula?
Pour 500 ml of distilled water into a 2000 ml Erlenmeyer flask, dissolve the media components completely in the water in the order of the formula, rinse the flask with the remaining 500 ml water, mix the medium thoroughly, adjust the pH, and sterilize.
What should you do if the medium contains agar?
If the medium contains agar, heat it until it starts to boil or even longer to completely dissolve the agar.
How can you maintain sterility while using culture tubes?
Sterility can be maintained by capping culture tubes with cotton plugs, plastic foam plugs, plastic or metal caps, or screw caps. These caps allow air into the culture tube while minimizing evaporation and preventing contamination.
How can culture broth be dispensed?
Culture broth can be dispensed using a pipetting machine, an automatic syringe, or a regular pipette.
Why is it important to use a flask that holds twice the volume of the media being prepared?
It’s important to use a flask that holds twice the volume of the media being prepared to prevent overflow during mixing and sterilization processes.
