Calibrating the Microscope Flashcards
How do you properly calibrate the eyepiece micrometer with a stage micrometer using the low power objective?
Align the zero line of the stage micrometer with the zero line of the eyepiece micrometer, then scan until the lines align again. Determine the value that each line represents in the eyepiece micrometer.
What is the formula to calculate the calibration factor (CF) for the microscope?
Calibration factor (CF) = (Stage Micrometer division subtended by Ocular Micrometer) × (Value of one Stage Micrometer division) ÷ (Ocular Micrometer division subtended by Stage Micrometer).
How should you observe different prepared slides using the low power objective (LPO)?
Keep the eyepiece micrometers in place and observe the slides. Use the scanner for bigger specimens and the high power objective (HPO) for smaller ones, but ensure the objectives are calibrated first.
What should you do with the eyepiece micrometer and the calibration factor when measuring cells and organelles?
Use the eyepiece micrometer along with the calibration factor to measure the length of cells and distinct organelles. If the eyepiece micrometer is not available, estimate measurements using the computed field of view (FOV).
How should you document the measurements of cells and organelles observed under the microscope?
Document, label, and indicate the measurements. Include a ruler bar drawn to scale at the bottom of the drawing, with a line indicating a length of 10 microns. Also, include the magnification used for each observation.
What is the length of the stage micrometer and how many divisions does it have?
The stage micrometer is 1 mm long with 100 divisions.
What is the value of each division on the stage micrometer?
Each division of the stage micrometer is one one-hundredth of a mm (0.01mm or 10 µm).
How many units is the eyepiece micrometer divided into?
The eyepiece micrometer is divided into 100 units.
How do you determine the calibration factor using the eyepiece and stage micrometers?
The calibration factor is determined by aligning the zero marks of both micrometers, scanning until they align again, and then counting the divisions between them.
What is the purpose of including a ruler bar in microscopy documentation?
A ruler bar drawn to scale provides a reference for the size of observed objects, with a line indicating a specific length (e.g., 10 microns).
