Glyolysis Flashcards
PFK1 structure
4 identical subunits
Quaternary structure
Bond by NON covalent bonds
When PFK1 is tense
Less active
Had ATP bound
When PFK1 is relaxed
More active
Has ADP bound
PFK2 structure
1 subunit with FBpase attached
Tertiary structure
Hexokinase IV
Liver
Operates in HIGH glucose levels
HK 1/2/3
Muscles
Has higher Km =more sensitive
What inhibits Hk
G6P
Therefore inhibition of PFK1 = high G6P
Therefore inhibits HK
What does pyruvate kinase do?
What step
PEP —> pyruvate
10
What does phosphorylation of PK do
Inactivates it
What causes inactivation of PK
High glucagon (phosphorylation)
What causes activation of PK
F16BP
De phosphorylation
What’s the role of glucogenogensis
Where does this occur
Synthesise glucose from non carb sources
Important during starvation/muscular activity
Mainly in liver/kidneys
Where are all enzymes found
What 2 exceptions
Cytosol
Pyruvate carboxylase (mitochondria)
G6Phosphatase (ER)
High F2-6BP
Inhibits gluconeogenesis
Promotes glycolysis
Low F2-6BP
Inhibits glycolysis
Stimulates gluconeogenesis
