Glycolysis Part 2 Flashcards
Glycolysis “_____”
“Sweet Breaking”
Stage 1 of Glycolysis
Entrapment
Entrapment
Glucose being phosphorylated by Hexokinase
What type of binding does Hexokinase use?
Induced Fit
G6P to F6P is an example of a
reversible isomerization reaction
Entrapment Part 2
F6P is phosphorylated to F1,6P
Last Stage of Stage 1
Dihydroxyacetone phosphate(DHP) > Triose phosphate isomerase > Glyceraldehyde 3-phosphate (GAP)
TPI causes lethal anemia and neuronal problems why?
Red blood cells arent able to generate their own enerdy. rely on glycolysis
The purpose of Stage 1 is to
break down glucose 6C to two trioses in the cytoplasm
ATP count after Stage 1
2 ATP Used, 0 ATP made
First reaction of Stage 2
the formation of 1,3 bisphosphoglycerate (1,3BPG) by GAPDH
1,3 BPG is made exergonic through
Thioester Intermediate
1,3- BPG has a high level of free energy of
Hydrolysis/Phosphoryl Transfer Potential
Phosphoglycerate Kinase makes
2 ATP
Substrate level phosphorylation is so named because
the phosphate comes from the substrate and NOT ATP as usually occurs with kinases.
GAP (aldehyde)
product of triose phosphate isomerase (TPI)
3-PG (3-phosphoglycerate)
product of phosphoglycerate kinase
Pyruvate Kinase makes
2 ATP
PEP to Pyruvate Step drives
glycolysis
After Stage 2 of Glycolysis
2 ATP used, 2 Net ATP Made, 2 NADH Made, 2 Pyruvate
Glycolysis is thermodynamically…
favorable
Alcoholic Fermentation
microbes metabolize pyruvate to EtOH
hexokinase
catalyses first step of glycolysis and is allosterically inhibited by its product glucose 6-phosphate
phosphoglycerate kinase (PGK)
substrate level phosphorylation, up regulated in cancer cells due to hypoxia
phosphofructokinase (PFK)
catalyzes the most regulated step in glycolysis
High Energy Charge
HIGH ATP, turn off and on pathway either anabolic or catabolic
Low Energy Charge
LOW ATP,turn off and on pathways either anabolic or catabolic
