Glycolysis Part 2 Flashcards
Glycolysis “_____”
“Sweet Breaking”
Stage 1 of Glycolysis
Entrapment
Entrapment
Glucose being phosphorylated by Hexokinase
What type of binding does Hexokinase use?
Induced Fit
G6P to F6P is an example of a
reversible isomerization reaction
Entrapment Part 2
F6P is phosphorylated to F1,6P
Last Stage of Stage 1
Dihydroxyacetone phosphate(DHP) > Triose phosphate isomerase > Glyceraldehyde 3-phosphate (GAP)
TPI causes lethal anemia and neuronal problems why?
Red blood cells arent able to generate their own enerdy. rely on glycolysis
The purpose of Stage 1 is to
break down glucose 6C to two trioses in the cytoplasm
ATP count after Stage 1
2 ATP Used, 0 ATP made
First reaction of Stage 2
the formation of 1,3 bisphosphoglycerate (1,3BPG) by GAPDH
1,3 BPG is made exergonic through
Thioester Intermediate
1,3- BPG has a high level of free energy of
Hydrolysis/Phosphoryl Transfer Potential
Phosphoglycerate Kinase makes
2 ATP
Substrate level phosphorylation is so named because
the phosphate comes from the substrate and NOT ATP as usually occurs with kinases.
GAP (aldehyde)
product of triose phosphate isomerase (TPI)
3-PG (3-phosphoglycerate)
product of phosphoglycerate kinase
Pyruvate Kinase makes
2 ATP
PEP to Pyruvate Step drives
glycolysis
After Stage 2 of Glycolysis
2 ATP used, 2 Net ATP Made, 2 NADH Made, 2 Pyruvate
Glycolysis is thermodynamically…
favorable
Alcoholic Fermentation
microbes metabolize pyruvate to EtOH
hexokinase
catalyses first step of glycolysis and is allosterically inhibited by its product glucose 6-phosphate
phosphoglycerate kinase (PGK)
substrate level phosphorylation, up regulated in cancer cells due to hypoxia
