Glucose Metabolism Step 3

Question 1

Event One

Answer 1

G3P is phosphorylated to form 1,3 BPG

via G3PDH (GAPDHis commonly used)

NAD reduced to NADH in this step: “high energy electrons” called “EXPHOS”

Question 2

Event Two

Answer 2

1,3-BPG is oxidized to 3-PG

via Phosphoglycerate Kinase

which catalyzes substrate level phosphorylation of ADP

Question 3

Event Three

Answer 3

Phosphoglycerate moves phosphor bond from C3 to C2

forming 2-phosphoglycerate

Question 4

Event four

Answer 4

Enolase dehydrates 2-Phosphoglycerate to Phosphoenolpyruvate PEP

Question 5

Event five

Answer 5

irreversible step

PEP has high energy phosphoryl-transfer potential: donates phosphoryl group to ADP to form ATP, forming Pyruvate in the process

Question 6

Pyruvate Kinase regulatory mechanism

Answer 6

+stim: insulin, [fructose 1,6 BP]

-stim: Alanine, ATP, Glucagon

Question 7

Event 6 with oxygen

Answer 7

TCA cycle initiation

Question 8

Event 6 without oxygen

Answer 8

Pyruvate converted to lactate by lactate dehydrongenase:

NADH produced in first event in step 3 uses lactic acid to unload H; NAD recycled