Glucose Determination

Question 1

Catalyze

Answer 1

Glucose oxidase and Peroxidase

Question 2

Results Reporting: Above Normal

Answer 2

> 105 mg/dl

Question 3

40-75 mg/dl (2.22-4.17mmol/L)

Answer 3

CSF

Question 4

Glucose is oxidized in the presence of

Answer 4

Glucose oxidase (GOD)

Question 5

The hydrogen peroxide formed reacts, under the influence of ________ with ______ and 4-aminoantipyrine

Answer 5

Peroxidase (POD), phenol

Question 6

The intensity of the color is proportional to glucose concentration. What color and Complex is formed?

Answer 6

Red-Violet, Quinone Complex

Question 7

B-D-glucose+H2O+O2—Gluconic acid+H2O2

Answer 7

Primary Reaction

Question 8

Results Reporting: Normal

Answer 8

70-105 mg/dl

Question 9

Reagents: Phosphate Buffer

Answer 9

Maintain/Neutralize ph balance

Question 10

how many degrees are need to warm the tubes?

Answer 10

37°C

Question 11

What is As?

Answer 11

Absorbance of Standard

Question 12

Reagents: Color Developer

Answer 12

Phenol and 4-Aminoantipyrine

Question 13

H2O2+ 4aminoantipyrine+phenol— Quinone Complex+H2O2

Answer 13

Secondary Reaction

Question 14

Glucose Standard

Answer 14

100mg/dL

Question 15

How many minutes are need to warm the tubes?

Answer 15

5 minutes

Question 16

What is the meaning of Au?

Answer 16

Absorbance of sample

Question 17

What reference method used to detect glucose level

Answer 17

Hexokinase

Question 18

Reference Range: Serum/Plasma

Answer 18

70-105 mg/dL

Question 19

Results Reporting: <70mg/dL

Answer 19

Below Normal

Question 20

Zero spectrophotometer at _____ with _______(blank solution)

Answer 20

500 nm, distilled water