Glucose Determination Flashcards
Catalyze
Glucose oxidase and Peroxidase
Results Reporting: Above Normal
> 105 mg/dl
40-75 mg/dl (2.22-4.17mmol/L)
CSF
Glucose is oxidized in the presence of
Glucose oxidase (GOD)
The hydrogen peroxide formed reacts, under the influence of ________ with ______ and 4-aminoantipyrine
Peroxidase (POD), phenol
The intensity of the color is proportional to glucose concentration. What color and Complex is formed?
Red-Violet, Quinone Complex
B-D-glucose+H2O+O2—Gluconic acid+H2O2
Primary Reaction
Results Reporting: Normal
70-105 mg/dl
Reagents: Phosphate Buffer
Maintain/Neutralize ph balance
how many degrees are need to warm the tubes?
37°C
What is As?
Absorbance of Standard
Reagents: Color Developer
Phenol and 4-Aminoantipyrine
H2O2+ 4aminoantipyrine+phenol— Quinone Complex+H2O2
Secondary Reaction
Glucose Standard
100mg/dL
How many minutes are need to warm the tubes?
5 minutes
What is the meaning of Au?
Absorbance of sample
What reference method used to detect glucose level
Hexokinase
Reference Range: Serum/Plasma
70-105 mg/dL
Results Reporting: <70mg/dL
Below Normal
Zero spectrophotometer at _____ with _______(blank solution)
500 nm, distilled water
