Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Clinical Chemistry-MIDTERMS > 1 > Flashcards

1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Periodic Table flashcards Chemistry 101 flashcards AP® Chemistry flashcards Organic Chemistry 101 flashcards
1
Q

Method for Glucose Determination:
-Based on the phosphorylation of glucose to produce 6-phosphogluconate
-most specific enzymatic method

A

HEXOKINASE METHOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Method for Glucose Determination:
Based on the oxidation of B-D- glucose to D- gluconic- lactone and hydrogen peroxide

A

GLUCOSE OXIDASE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

INDICATORS OF GLUCOSE OXIDASE:
Aminoantipyrine + Phenol
quinone imine dye complex formation is measured at 510-540 nm
Commonly used in Dry Chemistry
Red Product

A

COLORIMETRIC GLUCOSE OXIDASE METHOD (Saifer Gernstenfield Method)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Electrochemical detection
Electrode measures oxygen consumption

A

GLUCOSE OXIDASE AND FERROCENE (POCT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Electrochemical detection
Electrode measures oxygen consumption

A

GLUCOSE OXIDASE AND FERROCENE (POCT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Measures the rate of oxygen consumption
Rate of consumption= concentration of glucose
It can be utilized for serum, urine, and spinal fluid but not in whole blood sample

A

POLAROGRAPHIC GLUCOSE OXIDASE METHOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The amount of NADH generated is proportional to the glucose concentration

A

GLUCOSE DEHYDROGENASE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Indicator: POCT: GDH

A

pyrroloquinoline-quinone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

POCT: GDH
yellow

A

Ferrocyanide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

POCT: GDH
Colorless

A

FERRICYANIDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

CHEMICAL METHODS:
Reduction of a yellow ferricyanide to a colorless ferocyanide by glucose
Inverse Colorimetry

A

FERRICYANIDE METHOD (HAGEDORN-JENSEN)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Chemical Method:
Condensation Method
Most Sensitive

A

ORTHO- TOLUIDINE (DUBOWSKI METHOD)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

ortho-toluidine: sample is mixed with ___________ acid and then heated

A

Trichloroacetic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Chemical Method: based on the reduction of Cu2+ ions to Cu+ ions in the presence of reducing sugars.

A

NELSON-SOMOGYI

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Chemical Method: based on the reduction of Cu2+ ions to Cu+ ions in the presence of reducing sugars.

A

NELSON-SOMOGYI

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Cu+ ions further reduced the ________________ complex

A

arsenomolibdate

17
Q

Reduction of arsenomolibdate complex produced a stable ______-colored dye and can be measured spectrophotometrically at ______nm

A

blue
500

18
Q

The arsenomolibdate complex is prepared by reacting ____________ and _____________ in sulfuric acid

A

ammonium molybdate
sodium arsenate

19
Q

based on precipitation of proteins by tungstic acid and reduction of the alkaline copper solution by blood sugar

A

FOLIN-WU METHOD

20
Q

The amount of ___________ ________ formed being estimated colorimetrically by reacting with _________________ ________

A

cuprous copper
phosphomolybdic acid

21
Q

The cuprous is oxidized to cupric and the phosphomolybdic acid reduced to ______________

A

molybdenum blue

Clinical Chemistry-MIDTERMS (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions